三七总皂苷对糖尿病肾病小鼠足细胞保护作用的机制研究(英文)  

Mechanism study of panax notoginseng saponins on protective effect for podocyte in diabetic nephropathy mice

作　　者：周静鑫[1] 艾志敏[2] 孙文[1] 吴丽丽[1] 秦灵灵[1] 李佳[1] 段颖[3] 郭翔宇[1] 敬珊珊[1] 郭璇[1] 吴欣莉[1] 刘铜华[1] 

机构地区：[1]北京中医药大学,北京100029 [2]达州市中西医结合医院,达州635000 [3]北京同仁医院,北京100730

出　　处：《中华中医药杂志》2014年第5期1316-1321,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：National Major Scientific and Technological Special Project for Signaficant New Drugs Development(No.2011ZX09101-004-04);Innovation Team Project of Beijing University of Chinese Medicine(No.2011CXTD-19);Ministry of Education Key Laboratory of Health Preservation of Chinese Medicine;Beijing Key Laboratory of Health Preservation of Chinese Medicine~~

摘　　要：目的:探讨三七总皂苷保护糖尿病肾病小鼠足细胞作用机制。方法:16只8周龄KK-Ay小鼠成模后分为模型组和三七总皂苷组,另8只C57BL/6J设为正常对照组。实验中每2周检测小鼠,血糖,每4周测定小鼠24h尿蛋白定量。治疗10周后检测小鼠肾功能指标血清肌酐及尿素氮,并称取小鼠肾脏,固定行小鼠肾组织HE、Masson、PAS染色,并制作WT-1免疫组化,结合软件分析计算小鼠肾小球足细胞数。Western Blot检测小鼠肾脏中nephrin,α3integrin和β1integrin蛋白表达,实时荧光定量PCR检测nephrin,α3integrin和β1integrin mRNA的表达。结果:与模型组相比,三七总皂苷组治疗10周后小鼠体质量、肾重、血糖、24h尿蛋白定量显著降低;光镜下显示有效改善肾小球系膜增生及保护足细胞数目。三七总皂苷组治疗后nephrin,α3integrin和β1integrin的蛋白表达量及mRNA表达量都明显高于模型组。结论:三七总皂苷可能通过调节nephrin,α3integrin和β1integrin而改善糖尿病肾病小鼠足细胞损伤。Objective： To explore the mechanism of panax notoginseng saponins （PNS） on the protective effect for podocyte in diabetic nephnopathy mice. Methods： Sixteen eight-week-old male KK-Ay mice were randomly divided into a vehicle group and a PNS group, while eight C57BL/6J mice were taken as a normal control group. Fasting blood glucose levels were measured every 2 weeks and 24h urinary samples were collected from each mouse every 4 weeks. After 10 weeks treatment, the biochemistry parameter, kidney weight and histological analyses including hematoxylin eosin （HE） staining, Masson＇s Trichrome, periodic acid-Schiff （PAS） stain, and immunohistochemical staining for WT-1 were measured. The protein and mRNA expressions of nephrin, a3integrin, and 131integrin were detected by Western Blot and RT-PCR. Results： Compared with the vehicle group, the PNS group decreased the body weight, kidney weight, blood glucose and 24h urine protein. PNS group ameliorated mesangial matrix expansion and capillary thickness and preserved the podocyte number marked with WT-1. In addition, the protein and mRNA expression of nephrin were significanly increased compared with the vehicle group. The tt3integrin, and 131integrin expression were higher than those in vehicle group. Conclusion： PNS attenuated the podocyte injury in the diabetic nephropathy of KK-Av mice.

关 键 词：三七总皂苷 糖尿病肾病 足细胞损伤 KK—Ay小鼠 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]