冠心康抗动脉粥样硬化作用机制与SDF-1/CXCR4生物轴失衡相关性的研究  

Correlation of Guanxinkang anti-atherosclerotic mechanism and SDF-1/CXCR4 biological axis imbalance

作　　者：秦合伟[1] 刘萍[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中华中医药杂志》2014年第5期1558-1562,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2012年度上海市优秀学术带头人计划项目(No.12XD1404700);教育部博士点基金资助项目(No.20133107110003);上海高级中西医结合人才培养项目(No.ZYSNXD012-RC-ZXY008)~~

摘　　要：目的:通过观察冠心康对ApoE-/-小鼠主动脉粥样斑块SDF-1、CXCR4及SDF-1 mRNA、CXCR4 mRNA表达的影响,揭示冠心康抗动脉粥样硬化作用机制与SDF-1/CXCR4生物轴失衡的相关性。方法:48只8周龄雄性ApoE-/-小鼠分为模型组、AMD3100组、联合组和冠心康组,每组12只;12只8周龄近交系C57BL/6/J小鼠作为正常组。各组于用药12周取材检测各组小鼠血脂(TC、TG、LDL-C、HDL-C)含量,外周血血小板表面PAC-1、CXCR4表达,观察主动脉病理损伤程度,检测主动脉或动脉粥样斑块内SDF-1、CXCR4表达含量及各组小鼠主动脉SDF-1、CXCR4基因表达水平。结果:冠心康能够调节血脂,抑制血液中PAC-1和CXCR4在血小板表面的表达量,抑制主动脉粥样斑块中的SDF-1、CXCR4及SDF-1 mRNA、CXCR4 mRNA表达,与模型组比较,差异有统计学意义(P<0.05);而AMD3100仅能抑制主动脉粥样斑块SDF-1、CXCR4表达及SDF-1 mRNA、CXCR4 mRNA表达,对血脂和血小板表面PAC-1和CXCR4的表达量影响不大。结论:SDF-1/CXCR4生物轴是冠心康干预ApoE-/-小鼠动脉粥样硬化形成的作用靶点之一,冠心康抗动脉粥样硬化是通过多靶点发挥作用的。Objective： To revealthecorrelation of Guanxinkang anti-atherosclerotic mechanisms with SDF-1/CXCR4 biological axis imbalance by examined the effects of Guanxinkang on SDF-1, CXCR4 expressing content, SDF-1, CXCR4 mRNA gene expression in the high-fat diet ApoE＋ mice. Methods： 48 male ApoE-s- mice were divided into model group, AMD3100 group, Joint group and Guanxinkang group; 12 inbred C57BL/6/J mice were used as the control group. Each group based on the 12 weeks of treatment in each group was to detect serum lipids （TC, TG, LDL-C, HDL-C） levels, peripheral blood platelet surface PAC-1, CXCR4 expressions, pathological extent of aortic injury, aortic or intra-arterial plaque SDF-1, CXCR4 expression levels and aorta SDF-1 gene, CXCR4 gene expression levels. Results： Guanxinkang could regulate cholesterol, reduce expression levels of PAC- 1 and CXCR4 in the platelet surface, inhibit expression of SDF-1, CXCR4, SDF-lmRNA and CXCR4 mRNA in the aortic plaque; Compared with the model group, the difference was statistically significant （P〈0.05）; while AMD3100 could inhibit expression of SDF-1, CXCR4, SDF-1 mR_NA and CXCR4 mRNA in the aortic plaque, but had little effect on expression levels of PAC-1 and CXCR4 in the platelet surface. Conclusion： SDF-1/CXCR4 biological axis is one target of Guanxinkang intervention ApoE-/- mice formation of atherosclerosis; Guanxinkang plays the role of anti-atherosclerosis by many targets.

关 键 词：冠心康 ApoE-小鼠 动脉粥样硬化 SDF-1 CXCR4生物轴 

分 类 号：R543.5[医药卫生—心血管疾病]