降糖消渴颗粒含药血清对C2C12细胞胰岛素抵抗的影响  

Effects of Jiangtang Xiaoke Granule containing serum on insulin resistance C2C12 IR cells

作　　者：赵丹丹[1] 穆倩倩 方心[1] 张东伟[1] 莫芳芳[1] 高思华[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2014年第5期1577-1579,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技重大专项子课题(No.2012ZX09103201-005);国家国际科技合作项目(No.2011DFA30920)~~

摘　　要：目的:研究降糖消渴颗粒含药血清(JGDS)对骨骼肌细胞胰岛素抵抗模型葡萄糖消耗量及葡萄糖摄取率的影响。方法:JGDS处理棕榈酸、小牛血清白蛋白(BSA)诱导而致胰岛素抵抗(IR)的C2C12骨骼肌细胞;通过检测培养液中的葡萄糖含量反映葡萄糖消耗量;2-NBDG葡萄糖摄入法观察细胞对葡萄糖的摄取率。结果:0.25mmol/L棕榈酸和1%BSA培养16h,使得C2C12骨骼肌细胞葡萄糖消耗量与葡萄糖摄取率显著下降(P<0.05),造成骨骼肌细胞IR模型,JGDS干预可使IR模型骨骼肌细胞葡萄糖消耗量显著增加,与正常血清相比,差异具有统计学意义(P<0.05),且该作用呈现一定的剂量依赖性;JGDS可增加IR骨骼肌细胞模型葡萄糖转运率,与模型组比较,差异具有统计学意义(P<0.05)。结论:降糖消渴颗粒含药血清能显著增加IR的C2C12骨骼肌细胞的葡萄糖消耗和摄取,这可能是降糖消渴颗粒改善骨骼肌IR的机制之一。Objective： To evaluate the effects of Jiangtang Xiaoke Granule containing serum （JGDS） on the glucose utilization and uptakes of C2C 12 muscle cell strains of insulin resistance （IR） model. Methods： The 1R of C2C 12 cells was induced by palmitate （PA） and bovine serum albumin （BSA）. Glucose consumption was observed by detecting the amount of glucose in the remaining medium and 2-[N-（7-Nitrobenz-2-oxa-l,3-diazol-4-yl）Amino]-2-Deoxyglucose; 2- NBDG was applied to detect the glucose uptake rate. Results： Cultivation in 0.25mmol/L palmitate and 1% BSA for 16 hours could induce glucose consumption and glucose uptake rate of C2C12 skeletal muscle cells to decrease significantly （P〈0.05）, which indicated the IR model was successfully constructed. The glucose consumption and uptake rate of the cells intervened by JGDS increased significantly compared with those administrated with normal blank serum （P〈0.05）, and the effect on the glucose consumption presented a dose dependent manner. Conclusion： JGDS can significantly increase the glucose consumption and uptake of C2C12 IR cells, which may be one of the mechanisms of Jiangtang Xiaoke in relieving IR.

关 键 词：降糖消渴颗粒 C2C12细胞 含药血清 胰岛素抵抗 葡萄糖消耗 葡萄糖摄取率 

分 类 号：R587.1[医药卫生—内分泌]