人型支原体ParE基因突变与耐氟喹诺酮类药物的关系  

Relationship between ParE gene mutation and fluoroquinolones resistance in Mycoplasma hominis

作　　者：罗永慧[1] 段颖卿[1] 甘雪华[1] 王旭菲[1] 舒向荣[1] 

机构地区：[1]江西省妇幼保健院,江西南昌330008

出　　处：《实验与检验医学》2014年第2期124-125,168,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生厅科技计划(项目编号:20123124)

摘　　要：目的探讨人型支原体对氟喹诺酮类药物的耐药机制。方法自临床分离的8株耐氟喹诺酮类药物的人型支原体(Mh),对其ParE基因PCR扩增后进行测序分析,与基因库中的野生型菌株MHPG21基因序列比对,分析ParE基因突变位点与菌株耐氟喹诺酮类药物的关系。结果与野生株MHPG21对比,6株检出ParE基因所编码的氨基酸残基发生D 426→N变异。结论 Mh临床分离株对氟喹诺酮类药物的耐药可能与ParE基因所编码的氨基酸残基D 426→N变异有关。Objective To explore the fluoroquinolones resistance meachanisms of Mycoplasma hominis （Mh）. Methods A total of 8 strains of Mh with different fluoroquinolones resistance phenotype were analyzed, the ParE gene was amplified and sequenced. The sequencing results of ParE gene were compared with that in the wild type strain PG21, then analyzed the relation between ParE gene mutation and fluoroquinolones resistance. Results Compared with wild strain PG21, ParE gene in 6 strains got a D426→N mutation. Conclusion The fluoroauinolones resistance of Mh might be associate with ParE gene D426→N mutation.

关 键 词：人型支原体 氟喹诺酮类药物 ParE基因 耐药性 

分 类 号：R446.5[医药卫生—诊断学] Q939.92[医药卫生—临床医学]