Effect of Different Concentrations of Lipopolysaccharide on Expression of NF-κB and LAP in Mammary Epithelial Cells of Dairy Cow  

作　　者：Cheng Lanling Cao Guifang Wen Shiyong Zhao Pengwei Tu Yong Jian Ruizhen Li Qi 

机构地区：[1]Histology and Developmental biology,Inner Mongolia Agricultural University

出　　处：《Animal Husbandry and Feed Science》2014年第1期17-21,共5页动物与饲料科学（英文版）

基　　金：Supported by National Natural Science Foundation of China(30460093,31060328);Projects for Key Areas of Scientific Research in Inner Mongolia Colleges and Universities(ZL010144);Major Projects for Cultivation of New Breeds of Genetically Modified Organisms(2008ZX08007-004)

摘　　要：The paper was to study the effects of different concentrations of lipopelysaccharide （LPS） on expression of nuclear factor Kappa B （NF-κB） and lingual antimicrobial peptide （LAP） gene in mammary epithelial ceils of dairy cow. The mammary epithelial ceils of dairy cow were stimulated by different concentrations （50, 100,200,400 and 800 ng/mL） of LPS. The total RNA of cells was extracted after stimulation for 2, 4, 8, 16, 24, 48 and 72 h, respectively, and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR. The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng/mL LPS for 72 h were the highest compared to the control group （ P 〈0.01 ）. The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of inammary epithelial cells induced by LPS, which regulated the expression of defense gene LAP, with certain dose and time effects.The paper was to study the effects of different concentrations of lipopelysaccharide （LPS） on expression of nuclear factor Kappa B （NF-κB） and lingual antimicrobial peptide （LAP） gene in mammary epithelial ceils of dairy cow. The mammary epithelial ceils of dairy cow were stimulated by different concentrations （50, 100,200,400 and 800 ng/mL） of LPS. The total RNA of cells was extracted after stimulation for 2, 4, 8, 16, 24, 48 and 72 h, respectively, and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR. The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng/mL LPS for 72 h were the highest compared to the control group （ P 〈0.01 ）. The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of inammary epithelial cells induced by LPS, which regulated the expression of defense gene LAP, with certain dose and time effects.

关 键 词：β-defensins Nuclear Factor kappa B Mammary gland epithelial cell LIPOPOLYSACCHARIDE 

分 类 号：S823[农业科学—畜牧学]