醋狼毒(月腺大戟)HPLC指纹图谱  

HPLC Fingerprints of Vinegar-processed Euphorbiae ebracteolatae Radix

作　　者：狄恒建[1] 庄果[2] 李俊松[2] 

机构地区：[1]江苏省食品药品检验所,南京210008 [2]南京中医药大学江苏省中药炮制重点实验室,南京210029

出　　处：《药学与临床研究》2014年第1期53-55,共3页Pharmaceutical and Clinical Research

基　　金：国家公益性行业专项基金项目(200807039);南京中医药大学中药学一级学科开放课题(2011ZYX2-011)

摘　　要：采用HPLC法,色谱条件:Welchorm-C18柱(4.6 mm×250 mm,5μm),在检测波长226 nm,柱温25℃下,以乙腈(A%)-水(B%)为流动相,流速1 mL·min-1梯度洗脱,初步建立了醋狼毒(月腺大戟)HPLC指纹图谱,确定醋狼毒(月腺大戟)中有24个共有峰。该方法准确、可靠、稳定,可以用于醋狼毒(月腺大戟)饮片的质量控制。Chromatographic fingerprints for the quality control of vinegar-processed Euphorbiae ebracte-olatae Radix were established on a Welchorm-C18 （250 mm×4.6 mm, 5μm） column with mobile phase ofacetonitrile and water by gradient elution at the flow rate of 1 mL·min-1. The column temperature was at 25℃ and detection wavelength was set at 226 nm. There were 24 common peaks selected in the chro-matograms, and all the common peeks were separated effectively. The precision, repeatability, and stabilityof this method were satisfying and can be used to identify and evaluate the quality of vinegar-processed Euphorbiae ebracteolatae Radix.

关 键 词：月腺大戟 指纹图谱 醋炙 

分 类 号：R927.1[医药卫生—药学]