西拉普利对大鼠肥厚心肌瞬间外向钾电流的影响  

Effect of cilazapril on transient outward potassium current in rats with ventricular hypertrophy

作　　者：黄美娇[1] 陈弹[1] 李红霞[1] 程绪杰[1] 李勋[1] 蒋庭波[1] 杨向军[1] 蒋文平[1] 

机构地区：[1]苏州大学附属第一医院心脏病科,江苏省215006

出　　处：《江苏医药》2014年第8期872-874,共3页Jiangsu Medical Journal

摘　　要：目的研究西拉普利对大鼠肥厚心肌瞬间外向钾电流(Ito)的电压门控钾通道α亚基(Kv4.2、Kv4.3)表达的影响。方法 30只大鼠随机均分为假手术(A)组、腹主动脉缩窄+安慰剂(B)组、腹主动脉缩窄+西拉普利(C)组。9周后,测量大鼠体重(BW)、收缩压(SBP)、心率(HR)、心脏重量(HW)、左心室重量(LVW)及左心室重量指数(LVW/BW),采用RT-PCR和Western blot法分别检测心肌Kv4.2、Kv4.3mRNA和蛋白表达。结果与A组相比,9周后B组大鼠SBP、HR、HW、LVW、LVW/BW增加(P<0.05),Kv4.2、Kv4.3mRNA和蛋白表达减少(P<0.01);而C组能有效逆转B组上述各指标的变化(P<0.05)。结论西拉普利能上调Kv4.2、Kv4.3表达,有效改善大鼠心肌肥厚。Objective To investigate the effect of cilazapril on the expressions of alpha subunits of voltage-gated potassium channels（Kv4. 2 and Kv4. 3） of transient outward potassium current in rats with ventricular hypertrophy. Methods Thirty rats were equally randomized into three groups of A （sham operated）, B（abdominal aortic coarctation followed by starch 2mg · kg-1 · d-1） and C （abdominal aortic coarctation followed by cilazapril 2 mg · kg-1 · d-1 ）. Body weight （BW）, systolic blood pressure （SBP）, heart rate （HR）, heart weight （HW）, left ventricle weight （LVW） and LVW/BW were measured after 9 weeks. The mRNA and protein expressions of Kv4. 2 and Kv4. 3 were detected by RT-PCR and Western blot, respectively. Results Compared with group A, the levels of SBP, HR, HW, LVW and LVW/BW in group B were increased（P〈0. 05）, while the rnRNA and protein expressions of Kv4. 2 and Kv4. 3 were decreased （P〈0. 01）, which were all significantly reversed in group C（P〈0.05）. Conehtsion Cilazipril can effectively upregulate the expressions of Kv4. 2 and Kv4. 3 and improve the ventricular hypertrophy in rats.

关 键 词：西拉普利 心肌肥厚 瞬间外向钾电流 

分 类 号：R331[医药卫生—人体生理学]