乌司他丁对脓毒症致大鼠急性肾损伤肾功能及尿相关指标的影响  

Protective effects of ulinastatin on acute kidney injury in rats with sepsis

作　　者：曹波[1] 张琳琳[1] 柴春香[1] 赵学兰[1] 张培荣[1] 陈学勋[1] 

机构地区：[1]潍坊医学院附属医院,山东潍坊261031

出　　处：《中国医药科学》2014年第5期25-28,共4页China Medicine And Pharmacy

基　　金：山东省潍坊市卫生局科研项目[(2013)年第078号]

摘　　要：目的探讨乌司他丁(UTI)对盲肠结扎穿孔术(CLP)所致大鼠脓毒症急性肾损伤(AKI)的保护作用。方法 SD健康雄性大鼠55只,按随机化原则分成3组:正常对照组5只、模型组25只、乌司他丁治疗组25只,后两组再随机分为5个时间点(1h、6h、12h、24h、48h),每组每时间点各5只,采用盲肠结扎穿孔术复制脓毒症模型,乌司他丁治疗组,造模后立即给予乌司他丁10万U/kg,尾静脉注射,分别在各个时间点采血、留尿标本,进行肾功能血肌酐(Scr)、尿素(Urea),尿肾损伤分子(KIM-1)、白细胞介素-18(IL-18)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)检测分析。结果与模型组相比,乌司他丁治疗组大鼠各时间点Scr、Urea,尿KIM-1、IL-18、NGAL浓度均显著降低,差异均有统计学意义(P<0.05)。结论乌司他丁对脓毒症所致急性肾损伤具有一定的肾脏保护作用。Objective To investigate the effects of ulinastatin on acute kidney injury in rats with sepsis. Methods Fifty-five healthy male sprague-dawley rats were randomly divided into 3 groups： normal control, model group, ulinastatin group.The rat models of sepsis were established by caecal ligation and puncture（CLP）.In treatment groups, rats were treated with ulinastatin through caudalis injection after CLP surgery.Each group was divided into 1, 6, 12, 24, 48 hour subgroups with 5 rats. Rats were sacrificed and tissue samples including kidney and blood samples were collected.The kidney function, urine level of kidney injury molecule-1 （KIM-1）, neutrophil gelatinase lipocalin（NGAL） and interleukin-18（IL-18） were measured. Results Compared with CLP model group, renal function, the levels of KIM-1, NGAL and IL-18 in urine, significantly decresed in Ulinasta group（P 〈 0.05）. Conclusion Ulinasta can improving renal function duringAKI in the rat model of sepsis.

关 键 词：乌司他丁 脓毒症 急性肾损伤 尿相关指标 

分 类 号：R96[医药卫生—药理学]