人源化NOD/SCID小鼠的细胞免疫重建  

Post-transplant cell immune reconstitution in humanized NOD/SCID mice

作　　者：杨海燕[1] 胡文华[2] 贾潇潇[2] 邓飞[2] 

机构地区：[1]安徽医科大学附属安庆市立医院病理科,安徽安庆246003 [2]遵义医学院,贵州遵义563000

出　　处：《细胞与分子免疫学杂志》2014年第5期533-536,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：贵州省科技厅(黔科合计省合2008-1003);遵义市"15851"人才工程项目(市15851人才办2008-9);贵州省教育厅培育项目(黔教科2009-113)

摘　　要：目的探讨人脐带血CD34+细胞移植于非肥胖性糖尿病/重症联合免疫缺陷(NOD/SCID)小鼠后的免疫重建作用。方法免疫磁珠法分选人脐血CD34+细胞,经外侧尾静脉移植于亚致死量照射的NOD/SCID小鼠。移植后4、6、8、10周流式细胞术动态监测小鼠外周血人源CD45+、CD3+、CD56+细胞的数量;10周后PCR检测小鼠骨髓人ALU基因的表达,免疫组织化学染色检测小鼠脾脏人源CD3+、CD56+细胞的表达。结果 NOD/SCID小鼠经照射后骨髓腔内有核细胞及巨细胞数量均明显减少或消失,达到理想的清髓预处理效果;移植后4、6、8、10周,移植组所有存活小鼠外周血均可检测到人源性CD45+、CD3+、CD56+细胞的表达,人源淋巴细胞的数量随时间的延长而变化,8周时达到高峰,10周仍有较高的比例。10周时移植组所有存活小鼠骨髓细胞均检测到人ALU基因的表达,脾脏均检测到人CD3+、CD56+细胞;未移植组小鼠照射后2周内全部死亡。结论经照射后的NOD/SCID小鼠通过移植人脐血CD34+细胞成功地建立了hu-SRC-NOD/SCID模型,并有效地重建了小鼠细胞免疫系统。Objective To investigate the cell irnnt~ reconstitution in non-obese diabetic/severe corrt3ined irnrnunode^cient （NOD/SCID） mice by the transplantation of human umbilical cord blood （HUCB） CD34 ＋ cells. Methods CD34＋ cells were isolated from HUCB by magnetic activated cell sorting （MACS）, and then were transplanted into NOD/SCID mice following the irradiation of sublethal doses via the lateral tail vein. Human CD45 ＋ , CD3 ＋ , CD56 ＋ cell populations in the peripheral blood of mice were dynamically analyzed by flow cytometry （FCM） 4, 6, 8 and 10 weeks after transplantation. After 10 weeks, the expression of human ALU gene was detected by PCR Jn the bone marrow of mice, and the expressions of human CD3 ＋, CD56 ＋ cells were examined by immunohistochemical staining in the spleen tissues. Results After irradiation, the nucleated cells and giant cells in the marrow cavity of NOD/SCID mice were reduced significantly or completely demolished. The effect of myeloablaUve pretreatment was ideal. Human CD45 ＋ , CD3 ＋, CD56 ＋cells were found by FCM in the peripheral blood of all surviving mice in transplantation group 4, 6, 8, and 10 weeks after the transplantation. The population of the human lymphocytes varied over time, peaked at the 8th week, and remained at a high level later. At the ]0th week, the human ALU sequence could be detected in the bone marrow of all surviving mice in transplantation group, and human CD3 ＋ , CD56 ＋cells could be observed in the spleen tissues. All mice which received no transplantation died within 2 weeks after irradiation. Condasion The hu-SRC-NOD/SCID model was successfully established in irradiation-induced NOD/SCID mice by the transplantation of HUCB CD34 ＋ cells, and its cell immune system was effectively rebuilt.

关 键 词：NOD SCID小鼠 CD34+细胞 移植 细胞免疫重建 

分 类 号：R392.4[医药卫生—免疫学] R331.2[医药卫生—基础医学]