空肠弯曲菌PEB1 DNA和PEB1蛋白联合免疫小鼠的效果评价  

Evaluation of immunological efficiency induced by Campylobacter jejuni PEB1 DNA combined with PEB1 protein in mice

作　　者：刘琳琳[1] 来卫东[1] 胡娜[1] 张维颉[1] 陈婷婷[1] 王法权[1] 顾润国[1] 

机构地区：[1]山东医学高等专科学校病原与免疫学教研室,山东临沂276002

出　　处：《细胞与分子免疫学杂志》2014年第6期576-580,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省高等学校科技计划项目(J12LK14);临沂市科技发展计划项目(201313063)

摘　　要：目的初步检测抗空肠弯曲菌感染外膜黏附蛋白(PEB1)核酸疫苗与蛋白疫苗联合免疫诱导小鼠免疫应答水平。方法通过灌胃免疫的方法,将制备的核酸疫苗pcDNA3.1(-)-PEB1及蛋白疫苗PEB1采用核酸初次免疫-蛋白加强免疫及分别单独免疫的方法免疫雌性BALB/c小鼠,以PBS及空质粒pcDNA3.1(-)对照,检测各组小鼠体液免疫应答及细胞免疫应答水平。末次免疫后第28天,对各组小鼠灌胃攻击空肠弯曲菌,评价疫苗保护率。结果免疫后第56天,核酸蛋白联合免疫组小鼠脾淋巴细胞刺激指数(SI)为2.625±0.275,脾细胞培养上清中IFN-γ含量为(258.92±13.472)pg/mL。血清IgG抗体滴度为(2.507±0.124)μg/mL,胃和小肠黏膜冲洗液sIgA抗体滴度为(80.351±5.769)ng/mL,脾细胞培养上清中IL-4(377.47±14.560)pg/mL水平均明显高于各对照组(P<0.05)。小鼠攻击试验显示:核酸蛋白免疫组的疫苗保护率为91.53%。结论PEB1核酸蛋白联合免疫组诱导的免疫应答水平和疫苗保护率高于单独核酸疫苗免疫组和单独蛋白疫苗免疫组。Objective To explore the improved immunological responses induced by an amino acid ABC transporter, permease protein PEBI DNA vaccine primer-protein boost immunization method against Campylobacter jejuni. Methods The DNA vaccine pcDNA3.1 （ -）-PEB1 and protein vaccine were prepared, respectively. The female BALB/c mice were intranasally immunized with the vaccines, PBS and pcDNA3. 1 （-） were used as controls. The humoral and cellular immunological responses were detected in female BALB/c mice that were challenged by Campylobacter jejuni at 28 days after the final immunization. Results In the DNA primer-protein boost group at day 56, the stimulation index （SI） of lymphocytes was 2.625 ＋0.275, serum IgG was （2.507 ±0.124） lag/mL, IL-4 in spleen supernatant was （377.47 ± 14.560） pg/mL, IFN-v in spleen supernatant was （258. 920 ± 13.472） pg/mL, and slgA in genital tract was （80.351 ±5. 769） ng/mL. All of them were significantly higher than those in controls （ P 〈 0.05）. The DNA primer-protein boost vaccines induced the strongest levels of protection to BALB/c mice （91.53%）. Conclusion The DNA primer-protein boost immunization could induce significant protective immunity against Campylobacter jejuni Challenge. It could significantly enhance both humoral and cellular immunologic responses in BALB/c mice, compared with DNA vaccine or protein vaccine immunization alone.

关 键 词：空肠弯曲菌 核酸疫苗 蛋白疫苗 联合免疫 

分 类 号：R392.11[医药卫生—免疫学] S852.43[医药卫生—基础医学]