扩增阻滞突变系统用于ABO血型基因分型研究  

ABO blood group genotyping based on amplification refractory mutation system

作　　者：张朝[1] 朱娟莉 杨江存[3] 马婷[3] 惠文利[1,2] 崔亚丽[1,2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]国家微检测系统工程技术研究中心,陕西西安710069 [3]陕西省人民医院输血科,陕西西安710068

出　　处：《细胞与分子免疫学杂志》2014年第6期643-646,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(31200749)

摘　　要：目的建立一种快速、低成本的ABO血型基因分型方法。方法设计6对引物,建立扩增阻滞突变系统(ARMS),对30例中国汉族无关个体外周血样本的ABO基因型进行鉴定,并与血清学方法和直接测序结果进行比较。结果 ARMS技术检测30例样本结果同血清学方法、直接测序结果吻合率达到100%。结论该研究建立的ABO血型基因型鉴定方法可快速检测出28种ABO基因型,具有一定的临床应用价值。Objective To develop a rapid, cost-effective and reliable method for ABO genotyping. Methods Six pairs of primers were designed for the amplification refractory mutation system （ARMS）. Thirty peripheral blood DNA samples, derived from Chinese Han unrelated individuals, were genotyped by ARMS. The results were compared with the corresponding data obtained from serological methods and DNA sequencing. Results The ABO genotyping results using ARMS were perfectly in line with the serological methods and DNA sequencing. Conclusion The method developed and optimized in this study is able to accurately and rapidly detect 28 kinds of ABO genotypes, indicative of a new approach applicable to clinical practice.

关 键 词：扩增阻滞突变系统 ABO血型 基因分型 

分 类 号：R446.1[医药卫生—诊断学] R457.11[医药卫生—临床医学]