降钙素基因相关肽参与外侧缰核对痛觉的调节作用  

Calcitonin gene related peptide in lateral habenula check pain of adjustment

作　　者：付华[1] 王颖[1] 杨胜男[1] 李聪[1] 王学斌[2] 

机构地区：[1]长春师范大学,长春130032 [2]山东临沂大学生命科学学院,临沂276005

出　　处：《中国免疫学杂志》2014年第4期449-453,共5页Chinese Journal of Immunology

基　　金：吉林省科技厅自然科学基金项目(20130101125JC);吉林省教育厅"十二五"重点基金项目(2013第246号);山东省自然科学基金项目(No.ZR2011CM030)

摘　　要：目的:探讨外侧缰核注射不同浓度的降钙素基因相关肽(Calcitonin gene-related peptide,CGRP)对大鼠痛觉调节的影响。方法:对大鼠的外侧缰核分别微量注射浓度为0.875、1.75、3.5和7 nmol/μl CGRP,采用热板和压板测试,分别测量大鼠5、10、15、20、30、45和60 min的后爪缩爪反应潜伏期(Hindpaw Withdrawal Latency,HWL),观察CGRP对大鼠的镇痛效果,并与注射生理盐水的对照组大鼠作比较。结果:注射CGRP以后,大鼠的后爪缩爪反应潜伏期与对照组相比明显延长,其中浓度为0.875 nmol/μl CGRP对大鼠左爪热刺激HWL有差异(P<0.05),对大鼠右爪热刺激HWL差异显著(P<0.001),对大鼠左爪机械刺激HWL有显著差异(P<0.001),而对大鼠右爪机械刺激HWL差异不明显(P>0.05);浓度为1.75、3.5和7 nmol/μl CGRP对大鼠左、右爪热刺激、机械刺激HWL均有显著性差异(P<0.001)。CGRP对正常大鼠的镇痛作用具有剂量依赖性,且在20 min时效果最为明显。结论:CGRP在外侧缰核内明显延长大鼠的HWL,即CGRP在外侧缰核具有镇痛作用。Objective：To observe the analgesic effect of the CGRP on the model rats of chronic pain. Methods： Saline and 4 different gradients of mixture（0. 875,1.75,3.5 and 7.0 nmal/μl） would be injected into the Lateral habenular complex. Using the Hot-plate Test and Randall Selitto Test to measure Hindpaw Withdrawal Lateneies that caused by harmful heat and mechanical stimula- tion representing the value of pain in 5,10,15,20,30,45,60 rain. The HWL was used as a basic value, compared with the group which injected into saline. Results： Compared with the NS control group, There were different in 0. 875 nmol/μl CGRP of left rats foot ther- mal stimulus HWL（ P 〈 0.05 ）, right foot hot stimulation of rat HWL significant difference （ P 〈 0.001 ）, left foot mechanical stimulation on rats HWL has significant difference（P 〈 0. 001 ） , But the right foot mechanical stimulation of rat HWL difference was not obvious （ P 〉 0.05 ） ; The concentration of 1.75, 3.5 and 7 nmol/μl CGRP to rats with left and right foot hot stimulation, Each of them gets the best pharmacodynamic in 20 minutes. Conclusion： Caleitonin gene related peptide significantly prolong rats in the lateral rein nuclear HWL. The calcitonin gene related peptide in the lateral rein nuclear has analgesic action.

关 键 词：降钙素基因相关肽 外侧缰核 微量注射 镇痛 

分 类 号：Q424[生物学—神经生物学]