长芦人参、西洋参中总皂苷及单体皂苷Re,Rg_1,Rb_1的含量测定  

Content determination of total saponins and monomer saponins in Re,Rg_1,Rb_1 in Long neck ginseng and long neck American ginseng

作　　者：姜婷[1] 刘婷婷[1] 李春雷[1] 任跃英[1] 骆长林 康岩 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林省通化百泉参业集团有限公司,吉林通化134000

出　　处：《人参研究》2014年第1期17-22,共6页Ginseng Research

基　　金：国家科技计划支撑项目:2011BAI03B00;人参规范化种植提升;系列产品综合开发及品牌培育研究

摘　　要：目的 通过测定比较普通人参、西洋参和长芦人参、西洋参的总皂苷及单体皂苷Re、Rg1、Rb1的含量,以阐明长芦人参、西洋参主要成分含量的品质特点.方法 采用比色法测定总皂苷含量;采用高效液相色谱法测定人参单体皂苷Re、Rg1、Rb1的含量.结果 比色法测定的线性范围为15.0～75.0μg,相关系数r=0.9929;平均回收率为100.09％,RSD为0.86％（n=6）.单体皂苷Re、Rg1、Rb1与生长年份成正相关.结论 长芦人参、西洋参的总皂苷含量高于普通人参、西洋参,单体皂苷含量随着年份的增长而增加.Objective ： By comparing common the content of total saponins and monomer saponins re, rg rb, in varieties and long neck varieties of ginseng and American ginseng , to undemtand the content in long neck of ginseng and American ginseng .Methods：colorimetric method to determine the total saponins. HPLC method to determine the monomer saponins re, rgl, rbl.Resuhs：the linear rangeof the colorimetric method for determination is15.0-75.0,The correlation coefficient r=0.9929,The average recovery is 100. 09%, RSD is 0.86%（n =6）.Monomer saponin re, rgl, rbl and growth year into positive correlationo Conclusion： the content of total saponins in long neck strain of ginseng and American ginseng is higher than common varieties, Monomer saponin content increased as the growth of the year.

关 键 词：人参 西洋参 人参皂苷Re、Rg1、Rb1 人参总皂苷 比色法 含量测定 

分 类 号：R284[医药卫生—中药学]