油桐油质蛋白Oleosin编码基因5个VfOLE转录本的克隆与表达分析  

Isolation and Expression Analysis on Vernicia fordii Oleosin Gene of Five VfOLE Isoforms

作　　者：吴庆珂[1] 杨素素[1] 汪阳东[1] 高暝[1] 陈益存[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2014年第2期233-239,共7页Forest Research

基　　金：浙江省自然科学基金重点项目(LZ13C160001)

摘　　要：植物种子中,油脂主要贮藏在一种分散的、相对稳定的亚细胞微滴"油体"中,油质蛋白Oleosin是油体结构组成和功能调节的最主要蛋白质,在种子成熟、休眠和萌发过程中发挥重要作用。通过油桐种仁EST文库构建与测序,获得了油桐Oleosin(OLE)蛋白编码基因的5个转录本VfOLE1、VfOLE2、VfOLE3、VfOLE4、VfOLE5,完整阅读框长度分别为474、465、414、414、429 bp。通过实时荧光定量PCR技术分析了5个VfOLE转录本在油桐不同组织以及种仁成熟过程中的表达变化。结果表明,5个VfOLE转录本在成熟的种子中特异高效表达,其它组织中微弱表达;5个VfOLE表达趋势与桐酸、桐油积累趋势一致,其中在桐酸快速合成、桐油快速积累时期,VfOLE1、VfOLE2表达迅速上升并且表达量显著高于VfOLE3、VfOLE4、VfOLE5。试验结果为进一步研究VfOLE基因在桐油合成中的生物学功能提供了参考。Plant lipid in oilseeds is generally stored in a dispersive and stable sub-cellular droplet ＂oil body＂.And oleosin protein,as the main structural protein of oil body,is responsible for its functional modification.Oleosin plays an important role in the process of oilseed maturation,dormancy and germination.In this study,five oleosin isoforms,named VfOLE1,VfOLE2,VfOLE3,VfOLE4,VfOLE5,were isolated from Vernicia fordii （tung tree）.Quantitative real time PCR method was employed to identify the expression mode of oleosin gene of the 5 isoforms,and the results showed that the five VfOLEs were all expressed exclusively in the oilseeds.Furthermore,the rule of the five VfOLEs expression in the tung tree developing seeds was in accordance with those of tung oil and α-eleostearic acid accumulation.In addition,the VfOLE1 and VfOLE2 expression increased immediately and higher than those of the other VfOLEs in tung oil and α-eleostearic acid biosynthesis.

关 键 词：OLEOSIN 油桐 基因表达 

分 类 号：S718.46[农业科学—林学]