黄瓜花叶病毒三个毒株对烟草细胞内防御酶系统及细胞膜通透性的影响  

THE EFFECTS OF THREE CUCUMBER MOSAIC VIRUS ISOLATES ON THE DEFENDANT ENZYMES AND CELL MEMBRANE PERMEABILITY IN TOBACCO CELLS

作　　者：王海河[1,2] 林奇英[1,2] 谢联辉 吴祖建[1,2] 

机构地区：[1]福建农业大学植物病毒研究所 [2]福建省植物病毒学重点实验室,福州350002

出　　处：《植物病理学报》2001年第1期43-49,共7页Acta Phytopathologica Sinica

基　　金：福建省科技厅资助项目! (99- Z- 193)

摘　　要：用黄瓜花叶病毒 (CMV) PE、M和 Xb3个毒株接种烟草 (品种 K3 2 6)后 ,按时间顺序测定与细胞防御系统有关的酶活性和受侵细胞膜通透性变化 :1)苯丙氨酸解氨酶 (PAL) :Xb毒株侵染初期导致酶活性明显升高、后期下降 ,M毒株导致酶活性一直保持在较高水平 ,而 PE毒株则导致酶活性下降。 2 )过氧化物酶 (POD) :PE和 M毒株均导致酶活性升高 ,Xb毒株影响不大。 3)超氧化物歧化酶(SOD) :Xb和 M毒株导致酶活性前期升高、后期活性下降 ,PE毒株引起酶活性前期略微升高、后期下降。 4 )多酚氧化酶 (PPO) :Xb毒株导致发病前期酶活性急剧升高、后期降低 ,M毒株导致酶活性初期降低、后期回升 ,PE毒株导致酶活性升高。 5)受侵细胞在发病初期的膜通透性均低于正常水平 ,不同毒株引起的膜透性变化与症状的严重度均有独特的规律。 3个毒株的侵染均可引起细胞内可溶蛋白浓度的改变。Tobacco plants (var.K 326 ) were inoculated with three isolates of cucumber mosaic virus, and the changes in the defendant enzyme activity and the membrane permeability of tobacco cells were detected after infection. For PAL, isolate Xb caused the increase of the enzyme activity at first, followed by a decrease. Isolate M caused an increased activity of PAL, while isolate PE resulted in a decrease of the enzyme activity. For POD, isolates PE and M increased POD activity whereas isolate Xb had no effect. For SOD, isolate Xb and M first caused a higher activity of SOD, then a lower one than the control. PE had a reversed result. For PPO, isolate Xb increased the activity of PPO rapidly first, then decreased it, which was exa ctly opposite to the results brought by isolate M. Isolate PE caused a higher activity of PPO all through the infection period. These three isolates all caused the decrease of permeability of cell membranes, but with distinctive patterns. In addition, the concentrations of the soluble proteins in the infected cells were also affected by the infection of the three isolates.

关 键 词：黄瓜花叶病毒 细胞膜通透性 防御酶活性 烟草 致病机制 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]