利用野生大豆染色体片段代换系定位百粒重QTL  

QTL Mapping for 100-seed Weight Using Wild Soybean Chromosome Segment Substitution Lines

作　　者：陈庆山[1] 蒋洪蔚[1,2] 孙殿君[2] 刘春燕[2] 辛大伟[1] 曾庆力[1] 马占洲[1] 胡国华[2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090

出　　处：《大豆科学》2014年第2期154-160,共7页Soybean Science

基　　金：教育部新世纪优秀人才支持计划(NECT-1207-01);黑龙江省自然科学基金重点项目(ZD201213);现代农业产业体系(CARS-04-02A);黑龙江省博士后基金(LBH-Z12035);中国博士后基金(2012M520030);黑龙江省高校长江后备支持计划项目(2014CJHB004)

摘　　要：利用野生大豆ZYD00006(供体亲本)与黑龙江省主栽品种绥农14(轮回亲本)所构建的高世代(BC3)染色体片段代换系130个株行进行QTL定位。采用基于单标记的方差分析方法检测到25个百粒重相关的SSR位点,为避免由于标记位点共分离而产生的假阳性结果,对方差分析得到的相邻位点进行代换作图分析,最终获得分布于大豆10条连锁群上的19个百粒重相关位点。其中有7个位点与已有研究结果完全一致;2个位点与已有研究结果位置相距0.9和4.6 cM;其余10个位点首次发现,推测是本套材料的特有位点;其中位点QSW-D1a-2加性效应3.6,QSW-H-2加性效应-2.1,片段长度均小于10 cM,可作为继续研究的首选位点。A chromosome segment substitution lines （BC3 ）including 130 lines was constructed by the cross of wild soybean ZYD00006 （donor parent）and cuhivar Suinong 14 （recurrent parent）. The QTL underlying 100-seed weight was identified u-sing ANOVA Method based on single marker with trait. Twenty-five SSR markers underlying 100-seed weight were detected with ANOVA method. To avoid the false positive of co-segregation markers, substitution mapping was used to verify the result of ANOVA method. Finally, Nineteen QTL underlying 100-seed weight were identified using two methods and those QTL distribu-ted on 10 linkage groups. Seven QTL were in full accord with known results;two QTL were somewhat different with known re-suits of O. 9 cM or 4.6 eM distance. Another 10 ones were first discovery of loci,which should be specific loci in the study. QSW-Dlα-2 and QSW-H-2 with 3.6 and-2.1 of additive effects,fragments length were less than 10 cM could be used as the first choice loci for further study. In this study, substitution lines which had similar genetic background were used to QTL map-ping. The result of QTL mapping is more credible because there is no interference of genetic background. Specific materials and important loci lay a foundation for further study on 100-seed weight QTL fine mapping and molecular assisted breeding.

关 键 词：大豆 染色体片段代换系 百粒重 QTL定位 

分 类 号：S565.1[农业科学—作物学]