大豆胞囊线虫病抗性基因相关分子标记对杂交后代抗性的鉴定效率  

Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny

作　　者：马岩松[1,2] 刘鑫磊[1] 栾晓燕[1] 王家军[1] 李进荣[1] 于佰双[1] 邱丽娟[2] 

机构地区：[1]黑龙江省农业科学院大聂研究所,黑龙江哈尔滨150086 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《大豆科学》2014年第2期173-178,共6页Soybean Science

基　　金：黑龙江省自然科学基金(C201020);哈尔滨市科技创新人才研究专项资金项目(2011RFXYN034);国家“十二五”科技支撑计划(2011BAD35B06);黑龙江省农业科技创新工程(2012ZD003)

摘　　要：大豆胞囊线虫病的鉴定受条件和方法的制约,一直是大豆抗病资源筛选和抗病品种选育的限制因素之一,与抗病基因紧密连锁的分子标记的发现,为抗病鉴定提供了高效而准确的鉴定方法。以抗大豆胞囊线虫病不同世代的育种分离群体为材料,采用人工接种鉴定和抗病基因型鉴定相结合的方法,探讨了标记基因型在不同分离世代的鉴定效率。结果表明:随着分离群体世代的增加,抗病个体在分离群体中所占比例呈上升趋势,分离世代的卡方测验表明抗病性由2个隐性基因控制;基于抗病基因Rhg1开发的Indel标记rhg1-I4和SCN_Res Bridge对表型的检测效率达到88.89%,显著高于Satt309(50.00%);随着育种群体世代的增加,标记检测效率有所提高。不同标记组合并没有提高标记的检测效率。结果证明基于抗病基因序列开发的功能性标记在大豆抗胞囊线虫病育种中有广泛的应用前景。The restriction of identification condition and method is one of the important limiting factors for SCN germplasm screening and breeding. Using molecular marker closely linked with resistant gene is an efficient and accurate method to identi-fy the resistance to SCN. In this study, we adopted several generation of segregation populations derived from hybridization be-tween SCN resistant and susceptible parents to evaluate the identification efficiency of molecular markers. The ratio of individu-als resistant to SCN in segregation population enhanced with the generation increasing. Chi-test showed the resistance to SCN was controlled by two recessive genes in segregation population. The detection efficiency of two Indel markers based on Rhgl sequence,rhgI-I4 and SCN_Res Bridge, was 88.89%, and higher than that of Satt309 （50.00%）. With the increasing of breeding generation ,the molecular markers detecting efficiency improved. The combination between different markers didn＇t add more accuracy. We could anticipate that the markers designed by resistant sequence were new pathway to accelerale the SCN resistance breeding.

关 键 词：大豆胞囊线虫病 抗性鉴定 基因型 检测效率 

分 类 号：S565.1[农业科学—作物学]