芪白平肺胶囊对慢性阻塞性肺疾病模型大鼠的保护作用  

Protective Effects of Qibai Pingfei Capsules on Chronic Obstructive Pulmonary Disease Model Rats

作　　者：张贺[1] 葛平[1] 王晓玉[2] 孟楣[1,2] 

机构地区：[1]安徽中医药大学研究生院,合肥230038 [2]安徽中医药大学第一附属医院/国家中医药管理局中药制剂三级实验室,合肥230031

出　　处：《中国药房》2014年第19期1741-1743,共3页China Pharmacy

基　　金：安徽高校省级自然科学研究项目(No.KJ2011Z218)

摘　　要：目的:研究芪白平肺胶囊对慢性阻塞性肺疾病(COPD)模型大鼠的保护作用。方法:采用复合因素法复制大鼠COPD模型。90只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、固本咳喘片(0.72 g/kg)组与芪白平肺高、中、低剂量(2.88、1.44、0.72 g/kg)组,复制模型第31天开始灌胃给药,每天1次,连续30 d。测定大鼠支气管肺泡灌洗液(BALF)中细胞总数和分类计数,测定BALF中白细胞介素(IL)-8、IL-6和肿瘤坏死因子(TNF)-α的含量。结果:与正常对照组比较,模型组大鼠BALF中白细胞总数增加,中性粒细胞总数、淋巴细胞比例、中性粒细胞比例增加,单核-巨噬细胞比例减少,IL-6、IL-8、TNF-α含量增加,差异有统计学意义(P<0.01);与模型组比较,芪白平肺胶囊高、中、低剂量组大鼠BALF中白细胞总数、中性粒细胞比例、淋巴细胞比例减少,单核-巨噬细胞比例增加,IL-6、IL-8、TNF-α含量减少,差异有统计学意义(P<0.01或P<0.01)。结论:芪白平肺胶囊能下调COPD模型大鼠支气管BALF中IL-8、IL-6、TNF-α的含量,减轻气道炎症反应而发挥治疗作用。OBJECTIVE： To study the protective effects of Qibai pingfei capsules on chronic obstructive pulmonary disease （COPD） rats. METHODS： COPD model rat was induced by complex factors. 90 SD rats were randomly divided into normal con- trol group （constant volume of normal saline）, model group （constant volume of normal saline）, Guben kechuan tablets group （0.72 g/kg）, Qibai pingfei capsules high-dose, medium-dose and low-dose groups （2.88, 1.44, 0.72 g/kg）. They were given rele- vant medicine intragastrically once a day since 31th day after modeling for consecutive 30 days. Total amount and differential count- ing of cells in BALF of rats both were determined. The contents of IL-8, IL-6 and TNF-α were determined in bronchus. RE- SULTS： Compared with normal control group, total amount of leucocyte in BALF of model group increased; and the proportion of neutrophile granulocyte and leukomonocyte increased while that of mononuclear macrophage decreased; the contents of IL-6 and IL-8 increased; there was statistical significance（P〈0.01）. Compared with model group, the total amount of leucocyte in BALF of rats decreased in Qibai pingfei capsule high-dose, medium-dose and low-dose groups; the proportion of neutrophile granulocyte and leukomonocyte decreased; the levels of IL-6, IL-8 and TNF-α of BALF in Qibai pingfei group was decreased, there was statistical significance（P〈0.01,P〈0.01）. CONCLUSIONS： Qibai pingfei capsule can down-regulate the levels of I L-6, IL-8 and TNF-α of BALF in COPD model rats; it play a therapeutic role on COPD by way of reducing the air way inflammatory reaction.

关 键 词：慢性阻塞性肺疾病 芪白平肺胶囊 白细胞介素6 白细胞介素8 肿瘤坏死因子Α 

分 类 号：R285[医药卫生—中药学] R563[医药卫生—中医学]