蛇床子素的止痒作用  

Antipruritic Effects and Primary Mechanism of Osthole

作　　者：罗玉鸿[1] 高植明 梁逊莹[2] 付凯[3] 雷冬晓 吴晓丹[3] 聂红[3] 

机构地区：[1]珠海市人民医院药学部,广东珠海519000 [2]珠海市妇幼保健院,广东珠海519000 [3]中药药效物质基础及创新药物研究广东省普通高校重点实验室/暨南大学药学院,广州510632

出　　处：《中国药房》2014年第19期1750-1753,共4页China Pharmacy

基　　金：珠海市科技计划项目(No.201311140042)

摘　　要：目的:研究蛇床子素的止痒作用。方法:小鼠面颊刮毛部位皮内注射10μl二盐酸组胺或二磷酸氯喹以复制组胺依赖性或非依赖性面颊瘙痒模型。模型小鼠随机均分为正常对照(等容含0.2%CMC-Na溶液)组、模型(等容含0.2%CMC-Na溶液)组、阳性对照[特非拉定(1.2 mg/kg)/醋酸地塞米松(0.08 mg/kg)]组与蛇床子素高、中、低剂量(40、20、10 mg/kg)组。观察小鼠瘙抓行为,光学显微镜观察小鼠皮肤病理学。结果:与组胺依赖性模型组比较,蛇床子素高、中、低剂量组小鼠搔抓次数减少,差异有统计学意义(P<0.01或P<0.05);与组胺非依赖性模型组比较,蛇床子素高剂量组小鼠搔抓次数减少,差异有统计学意义(P<0.05)。模型组小鼠在注射二盐酸组胺或二磷酸氯喹后的前5 min搔抓次数达到最多,随后逐渐减少。高、中、低剂量蛇床子素均不改变模型小鼠搔抓次数的时程变化趋势。各组小鼠面颊皮肤未见明显病理改变。结论:蛇床子素对组胺依赖性和组胺非依赖性瘙痒均有明显的改善作用,其中对组胺依赖性瘙痒的止痒作用更好,可能与其抑制组胺作用有关。OBJECTIVE： To study antipruritic effects and mechanism of osthole. METHODS： The histamine-dependent and in- dependent itch model was established by intradermal injection of histamine 10 μl or chloroquine on the shaved cheek of mice, re- spectively. The model mice were randomly divided into normal control group （constant volume 0.2% CMC-Na solution） model （constant volume 0.2% CMC-Na solution）group, positive control（histamine 1.2 mg/kg or chloroquine 0.08 mg/kg） groupand ost- hole high-dose, medium-dose and low-dose （40,20,10 mg/kg）groups. The itch-related behavior was observed, and the pathomor- phological changes of the cheek skin were observed by microscope. RESULTS： Compared with normal control group, the times of scratching increased in model group; there was statistical significance （P〈 0.01 ）. Compared with the histamine-dependent itch mod- el group,, the times of scratching decreased in osthole high-dose, medium-dose and low-dose groups; there was statistical signifi- cance （P〈0.01, P〈0.05）. Compared with the histamine-independent itch model group, the times of scratching decreased in osthole high-dose groups; there was statistical significance （P〈0.05）.The times of scratching reached the peak in first 5 min after injection of chloroquine and then decreased gradually. The time interval of chloroquine-induced scratching times had no change in osthole groups. But no obvious pathomorphologic changes of the cheek skin were observed in mice. CONCLUSIONS： Osthole can improve histamine-dependent and independent itch, and osthole can better relieve histamine-dependent itch, which is possibly related to the suppression of histamine.

关 键 词：蛇床子素 组胺 氯喹 行为学分析 组胺依赖性瘙痒 组胺非依赖性瘙痒 

分 类 号：R285.5[医药卫生—中药学] R758.31[医药卫生—中医学]