正交试验优选黑面神枝叶中总黄酮水提工艺  

Optimization of Water Extraction Technology of Total Flavonoids from the Leaves of Breynia fruticosa

作　　者：彭伟文[1] 王英晶[1] 梅全喜[1] 戴卫波[1] 吴飞[1] 

机构地区：[1]广州中医药大学附属中山医院,广东中山528400

出　　处：《中国药房》2014年第19期1763-1765,共3页China Pharmacy

基　　金：广东省建设中医药强省立项资助课题(No.2010073);中山市科技计划项目(No.20102A033)

摘　　要：目的:优选黑面神枝叶中总黄酮的水提工艺。方法:采用单因素试验考察提取方法、提取温度、提取时间、加水量及提取次数对总黄酮提取率的影响。在此基础上,以总黄酮含量为评价指标,采用正交试验优选提取时间、提取次数和加水量对总黄酮提取率的影响。结果:最佳总黄酮水提工艺为100℃下加热提取,加20倍量水,提取2次,每次1 h。该条件下,黑面神枝叶中提取的总黄酮含量为79.16 mg/g。结论:所选工艺合理、可行,可用于黑面神枝叶中总黄酮的提取。OBJECTIVE： To optimize the water extraction technology of total flavonoids from the leaves of Breynia fruticosa. METHODS：Single factor test was adopted to investigate the effects of xtraction method, extraction temperature,, extraction time, water amount and extraction times on extraction rate of total flavonoids. Using the content of total flavonoids as index, the effects of extraction time, extraction times and water amount on the extraction rate of total flavonoids were optimized by orthogonal de- sign. RESULTS： The optimal extraction technology was as follows： extraction temperature of 100 ℃, 20 folds of water, extracting for 2 times, 1 h for each time. Under above conditions, the content of total flavonoids from the leaves of B. fruticosa was 79.16 mg/g. CONCLUSIONS ： The technology is reasonable and feasible, and can be used for the extraction of total flavonoids from the leaves of B. fruticosa.

关 键 词：黑面神 总黄酮 提取工艺 单因素试验 正交试验 

分 类 号：R284.2[医药卫生—中药学] R283[医药卫生—中医学]