苦参生物碱类成分的HPLC指纹图谱研究  

Study on HPLC Fingerprints of Alkaloids from Sophora flavescens

作　　者：易远红 郝月莆 

机构地区：[1]成都中医药大学附属医院药剂部,成都610072 [2]成都中医药大学中药资源系统研究与开发利用国家重点实验室培育基地,成都611137

出　　处：《中国药房》2014年第19期1772-1774,共3页China Pharmacy

摘　　要：目的:建立苦参生物碱类成分的高效液相色谱(HPLC)指纹图谱。方法:色谱柱为Kromasil NH2(250 mm×4.6 mm,5μm),流动相为乙腈-乙醇(8∶1,V/V)-3%磷酸水溶液(梯度洗脱),流速为1 ml/min,检测波长为220 nm;采用《中药色谱指纹图谱评价系统》(2004A版)对3批样品进行相似度评价。结果:建立的HPLC指纹图谱中,苦参生物碱类成分各峰分离度较好,一共确定了17个共有峰,并对其中5个峰进行了定位指认;3批样品的相似度均>0.9。结论:该方法可得到精密度、重复性、稳定性较好的苦参生物碱类成分的HPLC指纹图谱,为苦参提取物的质量控制提供另一种检测方法。OBJECTIVE： To establish HPLC fingerprint of alkaloids from Sophora flavescens. METHODS： The determination was performed on Kromasil NH2（250×4.6 ram, 5 μm） column＇with mobile phase consisted of acetonitrile-ethanol （8： 1, V/V）-3% phosphoric acid （gradient elution） at the flow rate of 1 ml/min. The detection wavelength was set at 220 nm. The similarity of 3 batches of samples was evaluated by using TCM Chromatographic Fingerprint Evaluation System （2004 A edition）. RESULTS： In HPLC fingerprints, the chromatographic peaks of alkaloids from S. flavescens were well-separated, and there were 17 common peaks, among which 5 peaks were positioned and identified. The similarity of 3 batches was more than 0.9. CONCLUSIONS： HPLC fingerprint of alkaloids from S. flavescens can be obtained with good precision, reproducibility and stability, providing anoth- er detection method for quality control of alkaloids from S. flavescens.

关 键 词：苦参 生物碱 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]