RP-HPLC法测定不同产地黄芩中5种黄酮苷元成分的含量  

Content Determination of 5 Kinds of Flavonoid Aglycone in Scutellaria baicalensis from Different Sources by RP-HPLC

作　　者：颜梅[1] 徐菲拉[1] 徐斌[1] 何三民[1] 梅新路[1] 

机构地区：[1]金华市中心医院,浙江金华321000

出　　处：《中国药房》2014年第19期1782-1784,共3页China Pharmacy

摘　　要：目的：建立测定不同产地黄芩中5种黄酮苷元成分含量的方法。方法：采用反相高效液相色谱法。色谱柱为AgilentExtend．C18（150min×4．6mm，5μm），流动相为乙腈．0．1％磷酸溶液（梯度洗脱），检测波长为276nm，流速为1．0ml／min，柱温为35℃。结果：去甲汉黄芩素、黄芩素、汉黄芩素、白杨素、千层纸素A的进样量分别在0．0180～0．1080、0．3780N2．2680、0．0945～0．5670、0．0225～0．1350、0．0354～0．2124μg范围内与各自峰面积积分值呈良好的线性关系（r分别为0．9999、0．9995、0．9996、0．9995、0．9995）；精密度、重复性、稳定性试验的RSD〈3％；加样回收率分别为98．72％（RSD=2．5％，n=6）、101．80％（RsD=2．0％，n=6）、99．25％（RSD=2．3％，n=6）、98．71％（RSD=1．5％，n=6）、99．05％（RSD=1．2％，n=6）。结论：该方法操作简便、快速，结果准确，可用于黄芩中黄酮苷元成分的含量测定。OBJECTIVE： To establish the method for the content determination of 5 kinds of flavonoid aglycone in Scutellaria baicalensis from different sources. METHODS： RP-HPLC method was adopted. The determination was performed on Agilent Ex- tend-Cts（ 150 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.1%phosphoric acid （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set at 276 nm, and column temperature was 35 ℃. RESULTS： The linear ra- nges of norepinephrine wogonin, baicalein, wogonin, chrysin and oroxylin-A were 0.018 0-0.108 0 μg （r： 0.999 9）, 0.378 0-2.268 0 μg（r=0.999 5）, 0.094 5-0.567 0 μg（r：0.999 6）,0.022 5-0.135 0 μg（r：0.999 5） and 0.035 4-0.212 4 μg（r：0.999 5）. RSDs of precision, reproducibility and stability tests were all lower than 3%. The recovery rates were 98.72% for norepinephrine wogonin （RSD：2.5% ,n=6）, 101.80% for baicalein （RSD=2.0% ,n：6）, 99.25% for wogonin （RSD=2.3% ,n=6）, 98.71% for chrys- in （RSD=l.5% ,n：6） and 99.05% for oroxylin-A （RSD=l.2% ,n=6）. CONCULSIONS： The method is simple, rapid and accu- rate, and it is feasible for content determination of flavonoid aglycone in S. baicalensis.

关 键 词：黄芩 黄酮苷元 含量测定 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]