柯萨奇病毒-腺病毒受体表达、病毒感染与心肌病变之间的关系  

The relationships among coxsackie-adenovirus receptor expression, viral infection and myocardial lesions

作　　者：魏春燕[1] 武茜 吴秀霞 

机构地区：[1]山东省莱芜市人民医院心内科,山东莱芜271100 [2]山东省莱芜市莱城区人民医院内科

出　　处：《国际病毒学杂志》2014年第2期79-81,共3页International Journal of Virology

摘　　要：目的 探讨和分析心肌组织柯萨奇病毒-腺病毒受体（coxsackie-adenovirus receptor,CAR）表达与病毒持续感染导致心肌病变的关系.方法 以扩张型心肌病（dilated cardiomyopathy,DCM）和慢性心肌炎患者的心肌组织标本作为病例组,以医院提供的先心病和冠心病等非感染性心脏疾病心肌组织及正常心肌组织标本作为对照组,采取免疫组化技术对心肌的CAR表达进行检测,采取巢式聚合酶链反应对相关心肌组织中腺病毒（adenovirus,AV） DNA和柯萨奇B组病毒（Coxsackie B virus,CVB） RNA进行检测,比较两组CAR表达、AV-DNA和CVB-RNA检出率的差异.结果 病例组标本33份,对照组标本14份.病例组与对照组患者CAR在心肌组织中表达的免疫组化面积积分分别为0.0118 ±0.0034和0.0039±0.0026,两组间的差异有统计学意义（P＜0.05）.病例CVB-RNA的检出率为39.4％,对照组标本中未检出CVB-RNA.两组的差异有统计学意义（P＜0.05）.病例组AV-DNA检出率为12.1％,对照组AV-DNA检测结果均为阴性.两组的差异有统计学意义（P＜0.05）.结论 AV和CVB感染、CAR表达与DCM和慢性心肌病的发展有紧密的联系.Objective To investigate the relationship between myocardial coxsackie adenovirus receptor （CAR） expression and myocardial lesions caused by persistent viral infection. Methods Myocardial specimens of patient＇s with dilated cardiomyopathy （DCM） and chronic myocarditis were collected as case group specimens, while the myocardial specimens from normal people and non-infectious heart disease patients were collected as control group. The immunohistoehemistry was applied in examining the expression of CAR, nested polymerase chain reaction was used to detect adenovirus （AV） DNA and Coxsackie B virus （CVB） RNA. The differences in terms of CAR expression, AV-DNA, CVB-RNA were compared between two groups. Results A total of 33 and 14 specimens were included in case group and control group, respectively. The CAR immunohistochemieal area integration was 0. 0118 ± 0. 0034 and 0. 0039 ± 0. 0026 respectively in case and control group, the difference was statistically significant （ P 〈 0. 05 ）. The positive rate of CVB-RNA was 39.4% in case group and zero in control group. There was significant difference between them （P 〈 0.05 ）. The positive rate of AV-DNA was 12. 1% in case group, while AV-DNA was tested negative in control group, the difference was statistically significant （ P 〈 0. 05 ）. Conclusions There is association between CVB, AV infection and CAR expression with DCM and chronic myocarditis.

关 键 词：扩张性心肌病 慢性心肌炎 柯萨奇B组病毒 柯萨奇病毒-腺病毒受体 

分 类 号：R373[医药卫生—病原生物学]