蜈蚣三七纳米粉预防大鼠术后肠粘连的机制研究  

Study on Mechanism of Nano-powder of Wugongsanqi in Preventing Postoperative Intestinal Adhesion in Rats

作　　者：黄欣[1] 李一鸣[2] 徐福琴[1] 何明钎[1] 邴飞虹[2] 

机构地区：[1]深圳市龙岗区人民医院普外科,深圳518172 [2]湖北中医药大学药学院中药资源与中药复方重点实验室,武汉430065

出　　处：《世界中医药》2014年第4期468-469,474,共3页World Chinese Medicine

基　　金：湖北省教育厅科研计划重点项目(编号:D20101802);深圳市科技局项目(编号:201103249)

摘　　要：目的:探讨蜈蚣三七纳米粉预防大鼠术后肠粘连的作用机制。方法:Ellis法建立大鼠术后肠粘连模型,随机分为假手术组、模型组、阳性组(肌内注射地塞米松10 mg/kg)、蜈蚣三七高、中、低剂量组(分别灌胃450、225、112 mg/kg)。术前3 d开始给药,术后继续给药1周。末次给药后,取血清,ELISA法检测TNF-α、IL-1β和IL-6的表达,并进行粘连程度肉眼分级。结果:与模型组比较,蜈蚣三七纳米粉各剂量均能明显改善肠粘连程度(P<0.05或P<0.01),均可明显降低血清TNF-α、IL-1β和IL-6水平(P<0.05或P<0.01)。结论:蜈蚣三七纳米粉可抑制炎性反应因子的过度表达,减轻炎性反应,有效预防术后肠粘连的发生。Objective： To investigate the anti-inflammatory mechanism of nano-powder of Wugongsanqi （ NW）, the rhizome of Anemone flaccid, on postoperative intestinal adhesion in rats. Methods： Fifty SD rats were subjected to operation with Ellis method for establis- hing intestinal adhesion models, then randomly divided into 5 groups （ n = 10）, namely model, positive （ Dexamethasone, i.m. 10 mg/ kg）, NW high, medium and low dose group （p. o. 450,225 and 112 mg/kg, respectively）. Another ten normal rats were selected as control group. Drugs were given 3 days before the operation until 7 days after the operation. The intestinal adhesion was graded and the levels of TNF-α、IL-1β and IL-6 in serum were evaluated by ELISA. Results： NW evidently reduced the severity of postoperative adhe- sion （ P 〈 0.05 or P 〈 0. 01 ）, and significantly inhibited the levels of TNF-α、IL-1β and IL-6 in serum （ P 〈 0.05 or P 〈 0. 01 ）, com- pared with those of the model group. Conclusion： NW could effectively prevent the formation of postoperative intestinal adhesion by in- hibiting the expression of inflammatory cytokines and improving the inflammatory response.

关 键 词：蜈蚣三七 肠粘连 TNF-Α IL-1Β IL-6 

分 类 号：R285.5[医药卫生—中药学]