虎雪兰与朱砂兰、蕙兰正反交育种及种子无菌萌发与增殖研究  

Research on Aseptic Budding and Fast Propagation on Cymbidium tracyanum var huanghua × Cymbidium mastersii Interspecific Hybridization Seed

作　　者：孟英[1] 闻永慧 李慧敏[1] 周元超 李枝林[1] 

机构地区：[1]云南农业大学花卉研究所,云南昆明650201 [2]临沂市园林科学研究院,山东临沂276001

出　　处：《安徽农业科学》2014年第13期3812-3814,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30160074)

摘　　要：[目的]研究虎雪兰与朱砂兰、蕙兰正反交育种及种子无菌萌发与增殖。[方法]以虎雪兰、朱砂兰、蕙兰‘绿春兰’为亲本,采用正反交杂交法进行育种试验得到果实,对成熟的种子进行无菌萌发试验,并对种子萌发获得的小苗进行增殖研究。[结果]朱砂兰为母本获得杂交种子无菌萌发最适培养基为:1/2MS+0.5 mg/L BA+0.2 mg/L NAA+3%花宝1号+0.05%碳粉;最佳增殖及生长培养基为:1/2MS+1.0 mg/L 6-BA+0.5 mg/L NAA+0.5 mg/L KT+3%花宝4号+0.05%碳粉。蕙兰‘绿春兰’为母本获得种子萌发最适培养基为:1/2MS+1.0 mg/L 6-BA+0.5 mg/L NAA+3%花宝1号+0.05%碳粉;最佳增殖及生长培养基为1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA+0.5 mg/L KT+3%花宝4号+0.05%碳粉。[结论]该方法为虎雪兰的种质资源栽培提供了理论依据。[Objective] This paper aims to study the aseptic budding and fast propagation on Cymbidium tracyanum var huanghua × Cymbidium mastersii interspecific hybridization seed.[Method] Cymbidium tracyanum var huanghua,Cymbidium mastersii and Cymbidium faberi Rolfe ‘ Lv Chun Lan’ were employed as parents.The hybrid seeds were tested using direct crossing and reciprocal crossing,then germinated and compared with different medium in axenic culture.[Result] The results show that the best medium for the seeds of Cymbidium mastersii in axenic culture is 1/2MS ＋ 0.5 mg/L BA ＋0.2 mg/L NAA ＋ 3％ Hyponex 1 ＋ 0.05％ C,the best medium for multiplication culture and differentiation is 1/2MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L NAA ＋ 0.5 mg/L KT ＋ 3 ％ Hyponex 4 ＋ 0.05％ C.The medium for the seeds of Cymbidium faberi Rolfe ‘ Lv Chun Lan’ in axenic culture is 1/2MS ＋ 1.0mg/L 6-BA ＋0.5 mg/L NAA ＋3％ Hyponex 1 ＋0.05％ C,the best medium for multiplication culture and differentiation is 1/2MS ＋ 1.5 mg/L 6-BA ＋0.5 mg/L NAA ＋0.5 mg/L KT ＋3％ Hyponex ＋0.05％C.[Conclusion] This method provides a theoretical basis for the germplasm resource cultivation of Cymbidium tracyanum var huanghua ×Cymbidium mastersii.

关 键 词：虎雪兰 种间杂交 无菌萌发 增殖 不亲和性 

分 类 号：S682.31[农业科学—观赏园艺]