嗜水气单胞菌的分离与16S rDNA鉴定  

Isolation and 16S rDNA Identification of Aeromonas hydrophila

作　　者：柳桐[1] 王生奎[1] 孙亭亭[1] 马志亮[1] 秦波[1] 张以芳[1] 信爱国 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省畜牧兽医科学院,云南昆明650224

出　　处：《安徽农业科学》2014年第13期3907-3910,共4页Journal of Anhui Agricultural Sciences

基　　金：云南省应用基础研究重点项目(2011FA014);云南省科技创新人才计划项目(2011HB035);国家自然科学基金项目(31260598)

摘　　要：[目的]分离并鉴定鱼嗜水气单胞菌,并构建系统发育分析。[方法]从云南某鱼场采集发病鱼的病变组织进行细菌分离与培养,并对分离菌株进行初步鉴定、16S rDNA鉴定和系统发育分析。[结果]分离培养到1株细菌,命名为Ah1305。经表型鉴定、生化试验及16S rDNA系统发育分析,鉴定为嗜水气单胞菌。[结论]该研究可为快捷、准确检测鱼嗜水气单胞引起的疾病提供参考。[Objective] The aim was to isolate and identify Aeromonas hydrophila and establish the phylogeny.[Method] Lesion tissue of fish was collected from a certain farm in Yunnan Province,and then the isolated strain was identified by preliminary identification,16S rDNA identification and phylogenetic analysis.[Result] The bacteria were isolated and named as Ah1305.Through the morphological identification,biochemical test and 16S rDNA phylogeny analysis,it was identified as Aeromonas hydrophila.[Conclusion] The study can provide reference for quick and accurately test the disease caused by Aeromonas hydrophila.

关 键 词：嗜水气单胞菌 分离鉴定 16S RDNA 

分 类 号：S182[农业科学—农业基础科学] Q93-331[生物学—微生物学]