徐淮山羊PRLR和INHBA基因的多态性及其与产羔数的关联分析  

Study on PRLR and INHBA Gene Polymorphism and Its Correlation with Prolificacy of Xuhuai Goat

作　　者：张玉龙[1] 孙伟[1] 苏锐[1] 陈禄[1] 张向楠[1] 倪蓉[1] 花为华 周洪[3] 陈家振 储明星[5] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]镇江市农科所,江苏句容212400 [3]徐州市睢宁县林牧渔业局,江苏睢宁221200 [4]江苏省丰县绿缘养羊专业合作社,江苏丰县221741 [5]中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与种质创新重点实验室,北京100193

出　　处：《中国畜牧杂志》2014年第9期14-18,共5页Chinese Journal of Animal Science

基　　金：现代肉羊产业技术体系建设(CARS-39);科技部家养动物平台项目;国家星火计划重点项目(2012GA690003);江苏高校优势学科建设工程资助项目;江苏省农业科技支撑计划项目(BE2012331);江苏省工程技术研究中心项目(BM2012308)

摘　　要：本实验以徐淮山羊产羔性状作为研究对象,以波尔山羊作为参照群体,采用PCR-SSCP方法分析PRLR基因和INHBA基因在2个群体中的单核苷酸多态性(SNP),并分析其与徐淮山羊群体产羔数性状的关联性。结果表明:所设计的5对引物在波尔山羊群体中的扩增片段都没有多态性,在徐淮山羊种中,引物2和引物5的扩增片段有多态性、引物2有AA和AB 2种基因型,引物5有CC和CD 2种基因型。经测序得知,AB型与AA型相比,PRLR基因外显子10在第307 bp处发生A→G的突变,CD型与CC型相比较,INHBA基因在1042 bp处发生T→A的突变。PRLR基因的AA和BB基因型的产羔羊数性状的最小二乘均值以及INHBA基因的CC和DD基因型的产羔羊数性状的最小二乘均值差异均不显著(P>0.05)。研究结果初步表明,检测的PRLR基因位点及其INHBA基因位点对徐淮山羊高繁殖力没有显著影响。PRLR and INHBA ge ne were studied as candidate in Xuhuai goat and Boer goat, to detect the single nueleotide polymorphisms （SNP） of PRLR gene and INHBA gene in the two groups by PCR-SSCP to study the relationship between the gene and the prolificacy of Xuhuai goat. The results showed that： The products amplified by five primers displayed no polymorphisms in Boer goat, primer P2 and P5 displayed polymorphisms in Xuhuai goat. For primer P2, there were two genotypes （AA and AB）, there were also two genotypes （CC and CD） for primer PS. The sequencing results revealed that the genotype AB compared to AA genotype, the exon 10 of PRLR gene had one point mutation （A--~G） at 307 bp, the genotype CD of INHBA gene had one point mutation （T--~A） at 1042bp compared to CC genotype. The difference of the least squares means （LSM） for litter size between the genotype AA and AB of PRLR gene and the genotype CC and CD of INHBA gene was non-significant （P〉0.05）. These results preliminarily showed that the locus of PRLR and INHBA gene had no significant effect to the high prolificacy of Xuhuai goat.

关 键 词：PRLR基因 INHBA基因 多态性 产羔数 徐淮山羊 

分 类 号：S827.2[农业科学—畜牧学]