僵蚕氯仿部位的分离纯化及其抗惊厥活性  

Compounds Isolated and Purified from Chloroform Active Part of Bombyx batryticatus and Their Anticonvulsive Activities

作　　者：郭晓恒[1] 吴用彦 宋登敏[1] 严铸云[3] 刘涛[1] 

机构地区：[1]成都大学医护学院,四川成都610106 [2]太极集团重庆涪陵制药厂有限公司,重庆408000 [3]成都中医药大学药学院,四川成都610075

出　　处：《中国医药工业杂志》2014年第5期431-433,共3页Chinese Journal of Pharmaceuticals

基　　金：四川省中医药管理局重点资助项目(2003A07)

摘　　要：对僵蚕氯仿部位进行分离纯化并考察其抗惊厥活性。采用硅胶柱色谱分离氯仿部位;运用MS和NMR分析鉴定化合物结构。从僵蚕中分离鉴定了5个化合物,分别为β-谷甾醇、麦角甾-6,22-二烯-3β,5α,8α-三醇、白僵菌素、6,9-氧桥-麦角甾-7,22-双烯-3-醇及β-胡萝卜苷,其中6,9-氧桥-麦角甾-7,22-双烯-3-醇首次从僵蚕中获得。白僵菌素为环肽类化合物,抗惊厥试验验证结果显示其具有抗惊厥活性,可作为僵蚕质量控制的指标。The compounds from the chloroform active part of Bombyx batryticatus were isolated and their anticonvulsive effects were investigated. A silica gel column chromatography was used to isolate and purify the compounds and their structures were identified by MS and NMR analysis. Five compounds were identified as β-sitosterol, ergost-6,22-dien-3β,5α,8α-triol, beauvericin, 6,9-epoxy-ergosta-7,22-dien-3-ol and β-daucosterol. Among them, 6,9-epoxy-ergosta-7,22-dien-3-ol was isolated from Bombyx batryticatus for the first time. The anticonvulsive effects were tested and the results showed that beauvericin had anticonvulsive effect, suggesting beauvericin could be served as the internal detective index for the quality control ofBombyx batryticatus.

关 键 词：僵蚕 白僵菌素 分离纯化 抗惊厥活性 

分 类 号：R917[医药卫生—药物分析学]