人类白细胞抗原DQA1基因多态性与尘肺病发病相关性分析  

Correlation analysis on pneumoconiosis morbidity and the polymorphism of HLA-DQA1

作　　者：赵娜[1,2] 杨锡贵[1] 孙炳欣[3] 曹殿凤[3] 高萍[3] 张宝玲[3] 

机构地区：[1]山东省医学科学院,山东省肿瘤医院,山东济南250017 [2]济南大学,山东省医学科学院医学与生命科学学院,250000 [3]淄博市职业病防治院,淄博255000

出　　处：《中国职业医学》2014年第2期168-171,共4页China Occupational Medicine

摘　　要：目的：探讨人类白细胞抗原（ HLA）-DQA1位点基因多态性与尘肺病发病的相关性。方法采用1∶1配比的病例-对照研究方法，选取130例已确诊为尘肺病的患者作为尘肺组，另选130例与尘肺组年龄和累计接尘工龄相近、同性别、同民族、同一工作地点的健康接尘工人作为对照组。用多重聚合酶链反应-连接酶检测反应分型法，检测2组研究对象HLA-DQA1共6个位点的等位基因，并分析其与尘肺病的相关性。结果尘肺组HLA-DQA1＊0201和HLA-DQA1＊0601基因频率均高于对照组（14.62％ vs 8.85％，12.69％ vs 7.31％），而HLA-DQA1＊0101基因频率低于对照组（34.23％vs 43.08％），差异均有统计学意义（P＜0.05）。 Logistic回归分析显示，HLA-DQA1＊0101、HLA-DQA1＊0201和HLA-DQA1＊0601等位基因频率与尘肺病有关联（P＜0.05），优势比分别为0.688、1.764和1.844，95％可信区间分别为（0.482～0.981）、（1.018～3.055）和（1.019～3.336）。结论 HLA-DQA1＊0201和HLA-DQA1＊0601基因可能是尘肺病的易感基因，HLA-DQA1＊0101基因可能是尘肺病的保护基因。Objective To explore the correlation between pneumoconiosis morbidity and the polymorphism of Human leu -kocyte antigen DQA1（HLA-DQA1） genes.Methods With the ratio of 1∶1 case control study, 130 cases with pneumoco-niosis were selected as the pneumoconiosis group , and other 130 health workers exposed to dust with similar age , length of service , gender , ethnic and the same working place were selected as the control group .Multiple polymerase chain reaction-ligase detection reaction was used to detect 6 alleles in HLA-DQA1, and analysis on the correlation between pneumoconiosis and them was conducted .Results HLA-DQA1＊ 0201, HLA-DQA1＊0601 gene frequencies in pneumoconiosis group were significantly higher than those in control group （14.62%vs 8.85%, 12.69%vs 7.31%, P＆lt;0.05）.HLA-DQA1＊0101 gene frequency in pneumoconiosis group was lower than that in control group （ 34.23% vs 43.08%, P＆lt;0.05 ） . Logistic regression analysis showed that HLA-DQA1＊0101, HLA-DQA1＊0201 and HLA-DQA1 ＊0601 allele frequency were associated with pneumoconiosis morbidity （P＆lt;0.05）.The odds ratio values were 0.688, 1.764 and 1.844, and the 95% confidence interval values were 0.482-0.981, 1.018-3.055 and 1.019-3.336, respectively.Conclusion HLA-DQA1＊0201 and HLA-DQA1 ＊0601 allele may be the susceptible risk genes for pneumoconiosis , and HLA-DQA1＊0101 allele may be the protective gene against developing pneumoconiosis .

关 键 词：人类白细胞抗原-DQA1 基因多态性 尘肺病 相关性 多重聚合酶链反应 连接酶检测反应 

分 类 号：R135.2[医药卫生—劳动卫生]