SSR荧光标记毛细管电泳法与国家冬油菜区试指纹鉴定平台的构建  

Fingerprint identification platform of capillary electrophoresis detection with fluorescent SSR markers on national winter rapeseed varieties( lines) field trials

作　　者：许鲲[1] 李锋[1] 吴金锋[1] 谷铁城[2] 陈碧云[1] 高桂珍[1] 闫贵欣[1] 李俊[1] 乔江伟 汪念[1] 伍晓明[1] 

机构地区：[1]农业部油料作物生物学与遗传育种重点实验室,中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部全国农业技术推广服务中心,北京100026

出　　处：《中国油料作物学报》2014年第2期150-159,共10页Chinese Journal of Oil Crop Sciences

基　　金：农业部全国农业推广服务中心项目;国家"十二五"科技支撑项目(2011BAD35B09;2011BAD01B03-08)

摘　　要：为了油菜品种指纹检测的精确性及未来构建大规模油菜新品种指纹数据库,应用SSR荧光标记毛细管电泳检测法构建国家冬油菜区试指纹鉴定平台。以40对荧光引物对2012-2013年度163份国家冬油菜区试参试品种（系）进行分析。结果共检测到42个等位位点和131个等位变异。其中A基因组（n1～n10）检测位点25个,C基因组（n11～n19）检测位点17个。每个位点等位变异数从2到6不等,平均为3.02。42个检测位点的PIC值变化范围在0.10～0.69之间,平均值为0.36。其中引物BRGMS171的杂合度、PIC值分别高达0.67、0.70,可考虑作为以后区试杂交种纯度鉴定的核心标记。SSR位点的纯合度分析得出本年度18份常规种平均纯合度为81.9%,145份杂交种为57.9%。以131个等位变异计算品种（系）间DICE相似系数,163份品种（系）间平均遗传相似系数变幅为0.607～0.765,变幅最大的为品种（系）FC03（0.438～0.879）,变幅最小为品种（系）宜油21（0.611～0.806）。To improve the accuracy of rapeseed cultivars fingerprint detection and to construct a large- scale rapeseed new variety fingerprint databases in the future,a fingerprint identification platform was constructed by capillary electrophoresis detection method with fluorescent SSR markers in the national winter rapeseed varieties（ lines） field trials. 42 locus and 131 alleles were detected by 40 fluorescent SSR primers in 163 newly bred winter rapeseed（ Brassica napus L.） varieties（ lines）. 25 locus were detected in A genome（ n1- n10） and 17 locus were detected in C genome（ n11- n19）. Alleles were obtained from 2- 6 with average of 3. 02 at 42 locus. The polymorphic information content values（ PIC） ranged from 0. 1 to 0. 69,with a mean of 0. 36. Primer BRGMS171 was determined according to the heterogosite rate（ 0. 67） and PIC value（ 0. 7） which could be used as preferred core primer for hybrid purity identification. The average SSR locus homozygosity of 18 open lines was 81. 9%,and that of 145 hybrid varieties was 57. 9%. Dice similarity coefficient among 163 newly bred winter rapeseed varieties（ lines） were calculated by 131 alleles,with an average range of 0. 607 to 0. 765. The maximum variation degree was in FC03 with a ranged of 0. 438- 0. 879 and the minimum was in Yiyou21 with a range of 0. 611- 0. 806.

关 键 词：冬油菜新品种(系) SSR荧光标记 毛细管电泳法 

分 类 号：S565.403[农业科学—作物学]