大豆TPS基因家族全基因组鉴定、分类与表达分析  

Genome- wide identification classification and expression of TPS family genes in soybean

作　　者：谢翎[1,2] 汪章勋[2] 黄勃[2] 

机构地区：[1]安庆师范学院生命科学学院,安徽安庆246011 [2]安徽农业大学微生物防治省重点实验室,安徽合肥230036

出　　处：《中国油料作物学报》2014年第2期160-167,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31201568);安徽省高校省级自然科学基金(KJ2013B123;KJ2011B081);安庆师范学院科研启动项目(044-K05000000281)

摘　　要：利用大豆基因组数据库,通过生物信息学分析,鉴定并获得大豆TPS家族基因所有成员的全序列、基因结构和定位信息。利用序列比对对大豆TPS家族基因进行进化和分类分析,同时通过soybase大豆发育表达芯片数据库相关信息,对该家族基因成员的组织表达谱进行了检测。研究结果表明,大豆基因组中含有20个TPS家族基因成员。系统发育分析将这些TPS基因分成了两个亚家族。基因定位分析表明,这些成员基因分别分布于大豆的14条染色体上。启动子分析表明,全部大豆TPS家族基因的启动子区都含有逆境反应顺式作用元件。转录水平芯片数据分析同时显示,大豆TPS家族基因大多在花、根、根瘤等组织中存在优势表达。该研究结果将促进大豆TPS家族基因的功能研究与利用。Based on soybean genome database,soybean TPS family genes were identified and their sequences, structures and chromosome location were determined by bioinformatic method. TPS proteins were classified according to their structure and phylogeny relationship. The RNA- sequencing data of TPS family genes from the soybase database was used to analyze the expression pattern of these genes in different tissues and treatments. The results showed that a total of 20 TPS genes located on 14 chromosomes respectively,could be systematically identified from soybean genome and classified into two classes. And all of them had stress related cis- acting elements in their promoter regions. Transcription analysis revealed that there was predominant expression of most soybean TPS family genes in flower,root and nodule. These results will facilitate functional analysis and utilization of TPS genes in soybean.

关 键 词：大豆 TPS家族基因 全基因组鉴定 进化分析 差异表达 

分 类 号：S565.103[农业科学—作物学]