姜黄素纳米粒子抑制乳腺癌细胞MCF-7活性的光学检测  

Optical Measurement of Cell Viability Inhibited by Curcumin-loaded Nanoparticles in Breast Cancer Cell Line MCF-7

作　　者：罗志慧[1] 杨洪钦[1] 何逸鹏 彭亦如[1,2] 李晖[1] 谢树森[1] 

机构地区：[1]福建省光子技术重点实验室,医学光电科学与技术教育部重点实验室,福建师范大学,福建福州350007 [2]福建省高分子材料重点实验室,福建师范大学化学与化工学院,福建福州350007

出　　处：《福建师范大学学报（自然科学版）》2014年第3期43-46,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金重点资助项目(61335011);国家自然科学基金面上项目(21274021);长江学者和创新团队发展计划(IRT1115)

摘　　要：采用纳米材料两亲嵌段共聚物聚乙二醇-聚已内酯(MPEG-PCL)将姜黄素包裹形成一种新型姜黄素纳米粒子。然后基于光学显微成像技术,比较姜黄素和姜黄素纳米粒子抑制乳腺癌细胞MCF-7的活性.通过激光共焦显微成像观察和检测姜黄素在细胞中的荧光强度比较MCF-7细胞系对姜黄素和姜黄素纳米粒子的摄取效率.结果表明姜黄素纳米粒子抑制癌细胞活性效果比较明显(P<0.01),MCF-7细胞系对姜黄素纳米粒子的摄取量大约为未包裹姜黄素的4倍,研究结果有助于促进纳米药物的研发及其在癌症治疗中的应用.Nano materials of amphiphilic block copolymer MPEG-PCL were used to encapsulatecurcumin into novel nanoparticles （nano-curcumin） . Then, based on the optical mmroscopm ima- ging technique, we compared MCF-7 cell viability cultured with curcumin and nano-curcumin. Cel- lular uptake of curcumin and nano-curcumin in MCF-7 was determined by cellular fluorescence in- tensity observed and detected by laser confocal microscopy imaging. Results showed that the inbibi- tion effect of nano-curcumin to MCF-7 viabihty was more obvious （ P 〈 0. 01 ） and cellular uptake of nano-curcumin in MCF-7 was about four times than the curcumin. The results will be helpful for the development of nanodrugs and its application in cancer therapy.

关 键 词：姜黄素纳米粒子 乳腺癌细胞系MCF-7 细胞活性 激光共焦显微成像 

分 类 号：R318.51[医药卫生—生物医学工程]