原子力显微镜观察新布尼亚病毒HB29株结果的初步分析  

Preliminary Analysis of Morphology of Bunya Virus of Severe Fever with Thrombocytopenia Syndrome by Atomic Force Microscopy

作　　者：张丽萍[1] 王玉春[1,2] 赵勇[1,3] 马雪征[1] 甄维[1] 胡孔新[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]河南师范大学 [3]吉林大学

出　　处：《检验检疫学刊》2014年第2期58-61,共4页Journal of Inspection and Quarantine

基　　金：中国检验检疫科学研究院基本科研业务费专项资金资助项目(2012JK014);国家质检总局科技计划项目(2014IK039)

摘　　要：[目的]利用原子力显微镜对新布尼亚病毒进行观察。[方法]超速离心制备新布尼亚病毒HB29株,采用磷钨酸负染透射电子显微镜进行观察;制备新布尼亚病毒的原子力显微镜样片,在此基础上对病毒进行原子力显微镜观察,采用轻敲模式在大气常温下进行扫描成像。[结果]透射电子显微镜观察到新布尼亚病毒球形病毒粒子,并提供病毒二维图像,可见刺突结构;原子力显微镜则呈现了新布尼亚病毒三维图像,且可见病毒表面有凹凸不平的特征和边缘不平滑,同时获得表面粗糙度等可量化指标。两种方法最终得到相似的形态学结果。[结论]利用原子力显微镜首次观察到新布尼亚病毒的三维形态结构,与透射电镜观察相比,原子力显微镜是一种制样简单,观察直观的新型病毒形态学研究工具。The morphology of the severe fever with thrombocytopenia syndrome bunya virus（SFTSV） was investigated by the atomic force microscopy（AFM） . The ultracentrifuged SFTSV HB29 strains were negatively stained by phosphotungstic acid, and observed under the transmission electron microscopy （TEM）. The SFTSV sample was prepared according to an AFM protocol, and then the SFTSV image was scanned with tapping mode at room temperature. The two-dimensional TEM image revealed a classical spike like sphere structure. The three-dimensional AFM image showed the characteristics and the morphology of the SFTSV strains. The SFTSV particle was an irregular sphere with an uneven edge. The measurements of the uneven edge were obtained in this study. This is the first time to have got the three-dimensional morphological structure of SFTSV by AFM. AFM is a new research tool for viral morphological observation with the advantages of simple preparing, 3D direct-viewing and visible interfaceing.

关 键 词：新布尼亚病毒 形态学 原子力显微镜 

分 类 号：R373[医药卫生—病原生物学]