酶联免疫法检测大便幽门螺杆菌抗原方法学评价  

Evaluation of ELISA in helicobacter pylori stool antigen detection

作　　者：孙安民 王伟 

机构地区：[1]开封市中心医院,河南开封475099 [2]新乡市新医三附院,河南新乡453000

出　　处：《中国卫生检验杂志》2014年第8期1135-1136,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的评价酶联免疫吸附试验(ELISA法)检测大便幽门螺杆菌抗原(HpSA)的临床价值和可靠性。方法联合尿素酶试验和组织学检测法(实验室检测幽门螺杆菌的"金标准")对200例慢性胃炎患者进行检测,同时采用酶联免疫吸附试验(ELISA法)测定其大便的HpSA,计算ELISA法的阳性率、特异度、敏感度和准确率,并比较二者阳性率的差异。结果 ELISA法检测大便HpSA的准确率为97.5%,特异度90%,敏感度103%,阳性率77.5%。二者阳性率没有显著性差异(P>0.05)。结论 ELISA法检测大便HpSA,方法特异,敏感度高,准确可靠,标本易得,操作简单,适合于幽门螺杆菌感染的普查和诊断。Objective To evaluate the clinical value and reliability of enzyme linked immunosorbent assay（ ELISA） in detection of helicobacter pylori stool antigen（ HpSA）. Methods The urease test and histology detection（ the ＂ gold standard＂ in laboratory detection of helicobacter pylori） were combined to detect helicobacter pylori in 200 chronic gastritis patients. While HpSA was detected in the stool of these patients at the same time by enzyme-linked immunosorbent assay（ ELISA）,and the positive rate,specificity,sensitivity and accuracy rate of ELISA were calculated,then the positive rates of the two methods were compared. Results Using ELISA method,the accuracy rate was 97. 5%,the specificity was 90%,the sensitivity was 103%, the positive rate was 77. 5%. There was no significant difference between the positive rates of ELISA method and the gold standard method（ P〉 0. 05）. Conclusion ELISA method for HpSA detection is specific,highly sensitive,accurate and reliable, easy to operate. It is easy to collect samples and suitable for screening and diagnosis of helicobacter pylori infection.

关 键 词：酶联免疫吸附试验 大便 幽门螺杆菌抗原 方法学评价 

分 类 号：R446.61[医药卫生—诊断学]