重组结核分枝杆菌38KD-Rv1419特异性抗原在结核病诊断中的价值  

Value of recombinant mycobacterium tuberculosis 38KD-Rv1419-specific antigen in diagnosis of tuberculosis

作　　者：陈松[1] 钟春燕[1] 滕勇[1] 陈宝炳[1] 

机构地区：[1]杭州市第三人民医院,浙江杭州310009

出　　处：《中国卫生检验杂志》2014年第8期1148-1150,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的评价结核分枝杆菌38KD-Rv1419重组蛋白在结核病血清学诊断中的应用价值。方法以痰涂片、培养为对照,采用化学发光法检测,评价68例结核病病人、61例非结核病病人血清中抗38KD-Rv1419抗体水平;绘制受试者工作特征曲线(receiver operating characteristic curve,ROC曲线),确定其诊断结核病的临界值,并评价其诊断效能。结果结核病组抗38KD-Rv1419抗体表达水平为229791.9±63846.9,明显高于非结核呼吸病组的22987.5±5024.3(P<0.05)。38KD-Rv1419抗体检测的灵敏度(63.2%)明显高于痰涂片和培养(16.2%)(P<0.05)。结论结核分枝杆菌38KD-Rv1419重组蛋白具有较高的结核病血清学诊断价值。Objective To evaluate the application value of Mycobacterium tuberculosis 38KD-Rv1419 recombinant protein in serological diagnosis of tuberculosis. Methods With sputum smear and culture as control,chemiluminescent enzyme immunoassay was used to detect serum 38KD-Rv1419 antibody level in 68 TB patients and 61 non-tuberculosis lung disease patients. The receiver operating characteristic curve（ ROC curves） was drew to determine the critical value of 38KD-Rv1419 in tuberculosis diagnosis and evaluate the diagnostic performance. Results The 38KD-Rv1419 antibody expression level was（ 229791. 9 ±63846. 9） in TB group,significantly higher than that in non-tuberculosis respiratory disease group（ 22987. 5 ± 5024. 3,P 〈0. 05）. 38KD-Rv1419 antibody detection showed higher sensitivity（ 63. 2%） than sputum smear and culture（ 16. 2%,P〈 0. 05）. Conclusion Mycobacterium tuberculosis 38KD-Rv1419 recombinant protein has high value in serological diagnosis of TB.

关 键 词：结核分枝杆菌38KD-Rv1419 化学发光酶免疫分析法 血清学诊断 

分 类 号：R52[医药卫生—内科学]