OsDREB3大豆品系特异性巢式PCR及多重PCR检测方法的建立  

Detecting method for event-specific nested-PCR and multiple PCR of OsDREB3 soybean

作　　者：刘营[1,2] 张明辉[1,2] 甄贞[1,2] 李璐[1,2] 高学军[1,2] 

机构地区：[1]农业部转基因生物产品成分监督检验测试中心,哈尔滨150030 [2]东北农业大学农业生物功能基因重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2014年第4期7-11,共5页Journal of Northeast Agricultural University

基　　金：转基因生物新品种培育重大专项(2014ZX08004-002-002)

摘　　要：为建立转基因大豆OsDREB3巢式PCR检测方法和转基因大豆多重PCR检测方法,选择转基因大豆GTS40-3-2、A2704-12及OsDREB3 DNA作为多重PCR方法的待检测基因模板,成功实现3种转基因大豆、4组不同片段同时扩增和检测。结果表明,可视性好,检测下限达0.1%。转基因大豆OsDREB3巢式PCR方法所用引物满足品系特异定性检测方法要求,其检测灵敏度达0.005%。For the establishment of nested PCR detection method of transgenic soybeans OsDREB3 and multiplex PCR detection method of genetically modified soybeans, modified soybeans GTS40-3-2, trans- genic soybean A2704-12 and GM soy OsDREB3 DNA were selected as gene templates of multiplex PCR method. Three kinds of genetically modified soybeans and four different fragments amplification could be detected simultaneously, and the sensitivity of multiplex PCR detection method was 0.1%. GM soybean OsDREB3 nested PCR primers were used to meet the requirements of event-specific qualitative detection methods, and the detection sensitivity was 0.005%.

关 键 词：转基因大豆 OsDREB3 品系特异性 巢式PCR 多重PCR 

分 类 号：Q81[生物学—生物工程] S565.1[农业科学—作物学]