新疆泥火山群土壤真菌群落的DGGE分析  

Analysis of the fungi communities in mud volcano soils in Xinjiang using DGGE

作　　者：张亚平[1] 曹小妮[1] 路盼盼[1] 余鹏[1] 李建辉[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《东北农业大学学报》2014年第4期66-70,共5页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(30860001)

摘　　要：采用DGGE指纹图谱技术对新疆乌苏的泥火山区的土壤真菌群落18SrDNA进行电泳分离,通过Quantity one软件对图谱进行聚类分析,并对主要条带进行回收测序。DGGE图谱聚类分析显示,五月份表层土真菌的种类和数量丰富。群落结构在相同季节垂直分布上相似性较高,聚集到一个分支上;而不同季节土样真菌组成差异较大。特异条带的回收测序结果表明,泥火山真菌优势菌群为链格孢属真菌和多枝横梗霉属。新疆泥火山土壤真菌的多样性较高但数量偏少,受气候环境因素的影响较大,优势菌群受此影响较小。Reveal the bacterial community structure and dynamics in mud volcano soil in Xinjiang. 18S-rDNA-ragment DGGE （denaturing gradient gel electrophoresis） and clone sequence BLAST technologies were applied, quantity one software was used to map the dendrogram. The cluster analysis of DGGE profiles showed that the number of species of fungi were the most abundant in the surface soil of May and community structure during the same season of the vertical distribution of high similarity, clustered into a branch; and fungi in soil samples composed of different seasons were quite different. The recovery of specific bands sequencing result showed that mud volcano fungi as Altemaria dominant fungi were Alternaria tenuissima, sp and Lichtheimia ramosa, sp. The fungi polymorphism in mud volcano soil in Xinjiang was significant, but the quantity was few, and this diversity was vulnerable to ecological, climatic and other factors. But the dominant microorganisms affected less.

关 键 词：泥火山 18SrDNA DGGE 

分 类 号：Q938.1[生物学—微生物学]