运动心脏重塑过程中miRNA-350/JNK信号通路的动态变化  

Dynamic Variation of MiRNA-350 and JNK Signaling Pathway in Mice Cardiac throughout Cardiac Remodeling Induced to Exercise Training

作　　者：翟帅[1] 陈佩林[1] 

机构地区：[1]南通大学,江苏南通226007

出　　处：《体育科学》2014年第5期9-14,44,共7页China Sport Science

基　　金：南通大学航海医学研究所"特种医学"学科建设专项课题(HYS-2013-004)

摘　　要：目的:通过递增负荷跑台运动建立运动性心肌肥大模型,观察miRNA-350及其靶基因在8周递增负荷运动诱导的心脏重塑中的动态变化,以探讨运动心脏重塑的可能机制。方法:96只雄性C57BL/6小鼠随机分为对照组(C组)和递增负荷运动组(ILT组)。正式训练的第7周末,用超声心动图检测各组小鼠心脏结构和功能参数;用qRT-PCR检测运动心脏重塑过程中不同时相(0d、3d、7d、15d、29d和57d)miRNA-350的动态变化;分别用qRT-PCR和Western Blotting技术检测JNK mRNA和JNK蛋白的动态变化。结果:ILT组小鼠PWTS、PWTD、LVEDD、IVSTD和IVSTS等指标显著高于C组,EF无显著性差异;在运动心脏重塑过程中,ILT组小鼠心脏中miRNA-350的表达水平先快速增高再急剧下降,然后缓慢下降到与C组相当的水平;在递增负荷运动的0d、3d和7d,miRNA-350的靶基因JNK mRNA的表达水平没有显著变化,此后,JNK mRNA的表达水平呈上升趋势;在运动心脏重塑的初期,JNK蛋白的表达水平先显著下降再缓慢上升。结论:持续8周递增负荷跑台运动成功的诱导了小鼠心肌肥大;运动心脏重塑过程中miRNA-350、JNK mRNA和JNK蛋白呈动态变化,提示miRNA-350/JNK信号通路可能参与运动心脏重塑的调节。Objective：Cardiac hypertrophy model was induced by increasing load treadmill exer- cise, in order to observe the dynamic variation of miRNA-350 and target molecules in mice car- diac throughout a 8 week＇s increasing loading training, and explore the possibly mechanism of cardiac remodeling exercise training. Methods： 96 male C57BL/6 mice were randomly divided into 2 groups named control group and increasing load group. The cardiac structural and function parameters of all mice were determined by the ultrasonic cardiogram at the end of 7th week during the formal exercise training. The dynamic alternations of miRNA-350 during different time phases of cardiac remodeling induced to exercise training was determined by qRT- PCR. The dynamic expression of JNK mRNA and JNK protein was determined by qRT-PCR and Western Blotting technology. Results： There were significantly differences in the sizes of PWTS, PWTD, LVEDD, IVSTD and IVSTS between the C group and group ILT, but no difference in the eject fraction. At first, the expression of miRNA-350 in the cardiac of ILT group presented a sharp ascent and followed an acute decline, and then slowly down to a level similar to C group. At the day of 0,3 and 7 throughout increasing load training period, the expression of JNK mRNA, target gene of miRNA-350 had no remarkable change. Since then the expression level of JNK mRNA increased gradually. The expression of JNK protein decreased dramatically and then increased slowly in the course of cardiac remodeling induced by exercise. Conclusions：It indicated that a lasting 8 weeks＇ increasing load treadmill exercise training induced a cardiac hypertrophy successfully. It appeared that the expression of miRNA-350,JNK mRNA and JNK protein showed a dynamic alternations during exercise-induced cardiac remodeling, and implied that miRNA-350/JNK signaling pathways possibly involved in the regulation of exercise-induced cardiac remodeling.

关 键 词：运动 心脏重塑 miRNA-350 JNK 信号通路 动物实验 鼠 

分 类 号：G804.5[文化科学—运动人体科学]