Borna病病毒与精神分裂症的关系研究  

Study on association of Borna disease virus infection with schizophrenia

作　　者：张丽[1] 徐平[1] 赵洪新[2] 

机构地区：[1]遵义医学院附属医院神经内科,贵州遵义563003 [2]遵义医学院附属医院神经外科,贵州遵义563003

出　　处：《贵州医药》2014年第3期200-203,共4页Guizhou Medical Journal

基　　金：国家自然科学基金资助项目(NO:31160210)

摘　　要：目的探讨贵州省部分地区博尔纳病病毒(Borna diseoce vicus)在精神分裂症患者中的感染情况及与疾病发生的相关性。方法采用巢式逆转录荧光定量PCR法对来自于贵州省部分地区(遵义、毕节、铜仁)的30例精神分裂症(SCH)患者及100例健康人的外周血液单核细胞(PBMC)中BDV p24基因片段进行检测,对阳性产物进行纯化、克隆、测序,用软件对所测定的序列结果进行同源性分析。结果精神分裂症患者中BDVp24基因片段阳性率为6.7%(2/30);健康体检者中未检出,二者检出率比较无统计学差异(P>0.05)。精神分裂症患者测序结果与GenBank提供的strain V毒株、C6BV毒株、He80/FR病毒株进行比较,突变率均为3%,均有3个位点出现一致性沉寂突变。与1 766毒株进行比较,突变率为2%,有2个位点出现一致性沉寂突变,即与1 766毒株的亲缘关系最近。结论本研究发现30例精神分裂症患者中检出BDVp24基因片段阳性2例,而健康人中未检出,说明Borna病病毒感染可能与精神分裂症有关,两组间BDV检出率无统计学差异,可能与所采标本地区较局限或标本量少有关。Objective To study the infection of Borna disease virus（BDV）with schizophrenia pa- tients in parts of Guizhou province and to study the correlation between the diseases and BDV. Meth- yls BDV p24 fragment of peripheral blood mononuclear cells （PBMC） from 30 Schizophrenia （SCH） patients and 100 healthy donors were examined by quantitative fluorescence nested reverse tran- scriptase polymerase chain reaction. Then Gene sequence and amino acid sequence were analyzed for positive products. Results The positive rate of BDV p24 fragment in PBMC from the patients with SCH was 6.7~, then the healthy donors were tested negative . Compared with the healthy donors, the positive rate of BDV p24 fragment in PBMC from the patients with SCH there were no statistical significantly differences （P~〉0.05）. The mutation of the sequence from the BDV p24 fragment of the patients with SCH was 3~ when compared with the stain V . The mutation of the sequence of the BDV p24 was 3 ~ when compared with the C6BV. Compared with the H1766, the mutation of the se- quence was 2~, and compared with He80/FR , the mutation of the sequence was 3~. At all, it kept the closest relationship with the 1766 strains. Conclusion 2 cases were detected BDV p24 from the 30 parents with SCH,borna virus infection may be associated with schizophrenia. But there were no sta- tistical significantly differences in the BDV p24 positive rate between the two groups it may be that the samples were less and the areas were more limited.

关 键 词：病毒性脑炎 精神分裂症 博尔纳病毒 

分 类 号：R512.3[医药卫生—内科学] R749.3[医药卫生—临床医学]