Characterization of a full-length infectious clone of bovine foamy virus 3026  

作　　者：Tiejun Bing Hong Yu Yue Li Lei Sun Juan Tan Yunqi Geng Wentao Qiao 

机构地区：[1]Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University [2]Department of Molecular and Cellular Pharmacology, the Vascular Biology Institute, University of Miami School of Medicine [3]National Laboratory of Biomacromolecules,Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences

出　　处：《Virologica Sinica》2014年第2期94-102,共9页中国病毒学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (31070135, 31370182);the Tianjin Research Program of Application Foundation and Advanced Technology (12JCQNJC06100);New Century Excellent Talents in University (NCET-10-0508)

摘　　要：The biological features of most foamy viruses(FVs) are poorly understood, including bovine foamy virus(BFV). BFV strain 3026(BFV3026) was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou, China. A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells, and it exhibited more than 99% amino acid(AA) homology to another BFV strain isolated in the USA. Upon transfection into fetal canine thymus cells, the full-length BFV3026 clone produced viral structural and auxiliary proteins, typical cytopathic effects, and virus particles. These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.The biological features of most foamy viruses （FVs） are poorly understood,including bovine foamy virus （BFV）.BFV strain 3026 （BFV3026） was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou,China.A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells,and it exhibited more than 99％ amino acid （AA） homology to another BFV strain isolated in the USA.Upon transfection into fetal canine thymus cells,the full-length BFV3026 clone produced viral structural and auxiliary proteins,typical cytopathic effects,and virus particles.These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.

关 键 词：bovine foamy virus infectious clone SYNCYTIUM electron microscopy 

分 类 号：S852.65[农业科学—基础兽医学]