白藜芦醇对去卵巢大鼠腰椎骨密度及NO、NOS影响  

Effects of resveratrol on BMDs of lumbar vertebraes and serum contents of NO and NOS in ovariectomized rats

作　　者：肖本熙[1] 张燕军[1] 王端[1] 戚本华[1] 马文君[1] 李远红[1] 潘妹霞[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)营养科,广州510080

出　　处：《中国公共卫生》2014年第5期632-634,共3页Chinese Journal of Public Health

基　　金：广东省科技计划项目(2011B061300032)

摘　　要：目的探讨白藜芦醇(RES)对去卵巢大鼠腰椎骨密度及一氧化氮(NO)和一氧化氮合酶(NOS)影响。方法 48只SD大鼠随机分为6组,分别为假手术组、去卵巢组、己烯雌酚(0.03 mg/kg)、RES 5、15、45 mg/kg组,处理90 d后,观察各组大鼠腰椎骨密度、血清中NO含量和NOS浓度变化。结果与假手术组比较,去卵巢组大鼠第4、5、6腰椎骨密度[分别为(0.141 4±0.001 1)、(0.139 1±0.000 9)和(0.152 8±0.000 6)g/cm2]、血清中NO含量[(8.190 5±3.814 8)μmol/L]和NOS浓度[(51.284 6±6.975 9)U/mL]均降低,差异有统计学意义(P<0.05);与去卵巢组比较,RES 45 mg/kg组第5、6腰椎骨密度[(0.156 0±0.001 1)、(0.173 3±0.001 5)g/cm2]、血清中NO含量[(18.624 3±2.613 0)μmol/L]和NOS浓度[(66.579 5±7.924 4)U/mL]明显升高,差异有统计学意义(P<0.05),RES 5、15 mg/kg组大鼠血清中NO和NOS含量均升高,差异有统计学意义(P<0.05)。结论白藜芦醇对去卵巢后大鼠腰椎骨密度降低有抑制作用,其机制可能与增加血清中NO含量和NOS浓度有关。Objective To investigate the effects of resveratrol （RES） on bone mineral density （ BMD）, serum con- tents of nitrogen monoxid （NO） and nitricoxide synthase （ NOS ） in ovariectomized female rats. Methods Forty-eight Sprague-Dawley female rats were randomly divided into 6 groups ： sham operated group, ovariectomized （OVX） group, OVX supplemented with 0.03 mg/kg diethylstilbestrol group（DES） ,and OVX groups supplemented with RES at dosage of 5,15 and 45 mg/kg,respectively. The duration of exposure was 90 days. The BMD of lumbar vertebrae and serum con- tents of NO and NOS of the rats were measured at the end of the exposure. Results Compared with the sham operated group, B MD of the 4th,5th and 6th lumbar vertebrae （0. 141 4 ± 0. 001 1,0. 139 1 ± 0. 000 9 and 0. 152 8 ± 0. 000 6 g/cm2 ） and serum levels of NO（8. 190 5 ±3. 814 8 μmol/L） and NOS（51. 284 6 ±6. 975 9 U/mL） of the OVX group were significantly decreased （ P 〈 0. 05 for all ）. The BMD of the 5th （ 0. 156 0 ± 0. 001 1 g/cm2 ） and 6th （ 0. 173 3 ± 0. 001 5 g/cm2） lumbar vertebraes and serum contents of NO （18. 624 3 e 2. 613 0 μmol/L） and NOS （66. 579 5 ± 7. 924 4 U/mL） of 45 mg/kg RES group were significantly higher than those of the OVX group（ P 〈 0.05 ）. Compared with the OVX group ,the NO content and NOS content of DES at 5 and 15 mg/kg of OVX rats were significantly increased（ P 〈 0. 05 ）. Conclusion Resveratrol has an inhibitory effect on lumbar vertebrae BMD in ovariectomized female rats and the mechanism of the effect may be related to the increased concentration of NO and NOS.

关 键 词：白藜芦醇(RES) 去卵巢大鼠 骨密度 一氧化氮(NO) 一氧化氮合酶(NOS) 

分 类 号：R181.24[医药卫生—流行病学]