AMBN基因多态性与环境因素在氟中毒中交互作用  

Interaction of polymorphisms of ameloblastin and environmental factors in endemic fluorosis

作　　者：蒋苗[1] 牟李红[1] 王应雄[2] 晏维 焦永卓[1] 

机构地区：[1]重庆医科大学公共卫生学院流行病学教研室,重庆400016 [2]重庆医科大学基础医学院 [3]重庆市疾病预防与控制中心

出　　处：《中国公共卫生》2014年第5期664-666,共3页Chinese Journal of Public Health

基　　金：重庆市地质矿产勘探开发局课题(渝地勘2010-1)

摘　　要：目的探讨成釉细胞蛋白(AMBN)基因多态性与环境因素在燃煤型地方性氟中毒中的交互作用。方法采用1∶1病例对照研究方法,选取重庆市巫山县笃坪乡和奉节县兴隆镇2个氟病区人群作为观察对象:病例组121例、病区对照121人,并利用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)法对AMBN基因多态性进行分析。应用条件logistic回归模型分析重庆市人群中氟中毒发生的环境及遗传因素,根据交互作用指标判断该基因与环境的交互作用。结果抽样地区人群的尿氟水平(F)≥0.95 mg/L及AMBN基因的7号外显子(538_540delGGA)的-/-(GGA完全缺失)基因型与13号外显子(986C>T)的CT基因型是该人群氟中毒的危险因素,两者分别存在交互作用(OR=5.600,γ=1.543;OR=4.375,γ=1.296)。结论携带(538_540delGGA)-/-基因型、(986C>T)CT基因型与尿氟含量(F≥0.95 mg/L)在燃煤型氟中毒中存在一定交互作用,燃煤型氟中毒与遗传及环境因素交互作用有关。Objective To explore the interaction of polymorphisms of ameloblastin（AMBN） gene and environmen- tal factors in endemic fluorosis. Methods A case-control study was carried out and Duping township of Wushan county and Xinglong township of Fengjie county in Chongqing municipality were chosen as fluoride areas. The observation sub- jects were divided into a case group（ 121 ） and a control group（ 121 in the endemic areas）. Polymerase chain-reaction re- striction fragment length polymorphism （PCR-RFLP） was used to determine the polymorphisms of AMBN. Interaction indexes（＇V） were calculated to determine the type of gene-environmental interation. Conditioned logistic regression analy- sis was adopted in data analysis with SPSS. Results Urinary fluoride of t〉0. 95 mg/L was interacted with the genotypes （538 540delGGA） - / - of exon 7 （ odds ratio E OR ] = 5. 600, y = 1.543 ） and genotype （ 986C 〉 T） CT of exon 13 （ OR =4. 375 ,y = 1.296） in AMBN in the incidence of endemic fluorosis. Conclusion Genotypes of 538 -540 delGGA -/- and（986C 〉T）CT in AMBN may interact with urinary fluoride（ ≥0. 95 mg/L）in the incidence of coal-burning fluorosis.

关 键 词：氟中毒 多态性 成釉细胞蛋白(AMBN) 基因 交互作用 

分 类 号：R18[医药卫生—流行病学]