血管内皮生长因子、癌胚抗原及端粒酶联合检测对良恶性胸腔积液的鉴别诊断价值  

Differential diagnosis value of combined measurement of VEGF,CEA and telomerase between benign and malignant pleural effusions

作　　者：刘晓华[1] 娜娜[1] 刘亚丽[1] 赵霞[1] 伊志刚[1] 

机构地区：[1]武警内蒙古总队医院内一科,呼和浩特010040

出　　处：《武警医学》2014年第4期360-362,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探索胸腔积液中血管内皮生长因子(vascular endothelial growth factor,VEGF)、癌胚抗原(carcinoembryonic antigen,CEA)及端粒酶单项和联合检测在良恶性胸腔积液中的诊断价值。方法选取70例胸腔积液患者,包括恶性胸腔积液34例,良性胸腔积液36例。采用ELISA法检测VEGF和CEA在两组病例胸腔积液中的表达,端粒酶的表达采用PCRELISA法检测。结果 VEGF、CEA及端粒酶在恶性胸腔积液患者组中的表达明显高于良性组(P<0.05);在单项检测中,VEGF的敏感性最高73.53%,其阳性预测值最高83.33%;CEA的特异性最高88.88%;联合检测中VEGF+CEA的敏感性最高91.17%,CEA+端粒酶的特异性低于VEGF+CEA和VEGF+端粒酶。结论 VEGF、CEA及端粒酶在良恶性胸腔积液的鉴别诊断中具有较高价值,联合检测可提高诊断的敏感性。Objective To study the differential diagnosis value of combined measurement of VEGF, CEA and telomerase be- tween benign and malignant pleural effusions . Methods Seventy patients with p]eural effusions were observed, including 34 cases of malignant pleural effusions and 36 cases of benign effusions. VEGF and CEA levels in pleural effusion were detected by ELISA. Telom- erase activity was tested by PCR-ELISA. Results The expresions of VEGF, CEA and telomerase in the group of patients with malig- nant pleural effusion were significantly higher than those in the benign group （ P 〈 0.05 ）. In single-item detection, the sensitivity and positive predictive values of VEGF were highest （73.53% ,83.33% ）. The specificity of CEA was highest（ 88.88% ）. In combined examination, the sensitivity of VEGF ＋ CEA was highest, and the specificity level of CEA ＋ telomerase was lower than that of VEGF ＋ CEA and VEGF ＋ telomerase. Conclusions VEGF, CEA, and telomerase hare high value in the differential diagnosis of benign and malignant pleural effusions. Combined examination can improve the diagnostic sensitivity.

关 键 词：血管内皮生长因子 癌胚抗原 端粒酶 胸腔积液 

分 类 号：R561.3[医药卫生—呼吸系统]