柱前衍生高效液相色谱法测定瓜蒌皮中游离氨基酸含量  

Determination of Free Amino Acids in Pericarpium Trichosanthis by High Performance Liquid Chromatography with Precolumn Derivatization

作　　者：孔浩[1] 郭庆梅[1] 郭新苗[1] 王晓[2] 周凤琴[1] 徐翠翠[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东省分析测试中心,山东济南250014

出　　处：《山东中医药大学学报》2014年第3期254-256,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(编号:2011BAI06B06);山东省科技发展计划项目(编号:2011GSF11904)

摘　　要：目的:建立柱前衍生高效液相色谱法(RP-HPLC)测定瓜蒌皮中游离氨基酸含量的方法。方法 :采用异硫氰酸苯酯(PITC)为柱前衍生试剂,高效液相色谱法色谱柱为(Atlantis dC18 Column(3μm,4.6 mm×150 mm,Waters),流动相A:甲醇-乙腈(1∶2),流动相B:0.15 mol/L乙酸钠,流速1.0mL/min,梯度洗脱,柱温35℃,吸收波长254 nm。结果:各种氨基酸均能达到基线分离。结论:柱前衍生高效液相色谱法作为瓜蒌皮药材中游离氨基酸的测定手段之一,准确可靠。Objective：To establish the determination of free amino acids in pericarpium trichosanthis by high performance liquid chromatography（HPLC） with precolumn derivatization. Methods：With the technology of precolumn derivatization,along with high performance liquid PITC and a high-efficiency Atlantis dC18 column （3 Is.m,4.6 mm x 150 mm,waters）,with mobile phase being A ： methyl alcohol-acetonitrile （1：2）, and B ： 0.15 mol/L sodium acetate trihydrateat,flow rate of 1.0 mL/min,peaks were detected at 254 nm and column temperature being 35 ℃. Results：Various kinds of free amino acids were able to achieve baseline separa- tion. Conclusion：HPLC with precolumn derivatization is precise and reliable for the determination of free amino acids in pericarpium trichosanthis.

关 键 词：瓜蒌皮 游离氨基酸 柱前衍生高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]