舒郁胶囊对大鼠海马神经元5-HT3R激活后Ca^(2+)的影响  

Effect of Shuyu Capsule on the Ca^(2+) of Activated 5-HT3R in Rat Hippocampus Neurons

作　　者：宋春红[1] 葛庆芳[2] 张惠云[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355 [2]兖矿集团总医院,山东邹城273500

出　　处：《山东中医药大学学报》2014年第3期263-265,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划项目(编号:2011-001)

摘　　要：目的:观察海马神经元内5-羟色胺3受体(5-HT3R)激活后信号通路中Ca2+浓度的变化,探索复方舒郁胶囊治疗抑郁情绪的微观作用机制。方法:培养大鼠海马神经元,在中药血清药理学指导下进行实验:于培养基中添加5-HT3R激动剂激活其介导的信号通路,再以一定浓度舒郁胶囊和柴胡提取物含药血清孵育神经元。采用荧光探针Fluo-3/AM标记结合激光共聚焦显微镜技术测定海马神经元胞内[Ca2+]i。结果:激动剂激活5-HT3R信号通路,神经元胞内[Ca2+]i显著升高(P<0.01)。给予正常大鼠舒郁胶囊和柴胡提取物含药血清干预,再次激活信号通路,胞内[Ca2+]i与正常组相比无明显变化,较激动剂组显著降低(P<0.05)。结论:舒郁胶囊通过5-HT3R调控海马神经元胞内[Ca2+]i的变化,可能是其治疗抑郁情绪的作用机制之一。Objective：To observe change of calcium concentration in 5-hydroxytryptophan 3 receptor（5-HT3R） mediate signal pathway in hippocampus neurons,and to explore microscopic mechanisms of Shuyu capsule acting on depression emotion. Methods：Serum-free primary culture method was adopted for hippocampus neurons of rat. Under the guidance of the Chinese serum pharmacology：agonist was added in the culture medium to activate its mediated signal pathway,and a certain concentration of Shuyu capsule and saikos- aponin medicated serum were prepared to incubate neurons. Fluorescent Fluo-3/AM and confocal microscopy were used to survey calcium concentration. Results：After incubating with agonist activating 5-HT3R,the in- tracellular calcium concentration levels in the hippocampus neurons increased obviously compared with the normal group（P〈0.01）. If we make drug serum interfered 24 hours beforehand,the intracellular calcium concentration in neurons had no difference with the normal group even though we added agonist in the medium,which is much higher than the agonist group（P〈0.05）. Conclusion：Shuyu capsule regulates the levels of Ca〉 by activiting of 5-HT3R signaling pathway,which may be one of the mechanisms to treat de- pression emotion.

关 键 词：海马神经元 CA^2+ 舒郁胶囊 柴胡 大鼠 

分 类 号：R285.5[医药卫生—中药学]