外源性硫化氢对胰岛素抵抗脂肪细胞葡萄糖转运体4表达的影响  

Effects of extrogenous hydrogen sulfide on the expression of glucose transporter 4 in 3T3-L1 adipocytes with insulin resistance

作　　者：杨非柯[1] 刘竟芳[1] 陈伟[1] 何新平[1] 卢桂静[2] 

机构地区：[1]长沙市中心医院老年医学科,410004 [2]中南大学湘雅医院心血管内科

出　　处：《中国心血管杂志》2014年第2期144-148,共5页Chinese Journal of Cardiovascular Medicine

基　　金：长沙科技计划项目(K120749-31)~~

摘　　要：目的观察外源性硫化氢(H2S)对3T3-L1脂肪细胞胰岛素抵抗(IR)的影响,并探讨其机制。方法用高糖高胰岛素培养3T3-L1脂肪细胞,建立IR细胞模型,外源性H2S供体NaHS(10-5、10-4和10-3mol/L)处理IR 3T3-L1细胞12、24和48 h。MTT法检测细胞活力,葡萄糖氧化酶法检测培养液中的葡萄糖消耗量,2-脱氧-[3H]-D-葡萄糖摄入法检测葡萄糖的摄取。实时定量PCR和Western blot检测葡萄糖转运体4(Glut4)的表达。结果与对照组比较,IR模型组细胞葡萄糖消耗和摄取量以及Glut4 mRNA和蛋白的表达显著降低(均为P<0.05)。与对照组比较,所有浓度的NaHS均未影响细胞活力。与IR模型组比较,NaHS(10-4和10-3mol/L)处理24和48 h显著增加细胞葡萄糖消耗和摄取量以及Glut4 mRNA和蛋白的表达(均P<0.05)。结论外源性H2S改善了高糖高胰岛素诱导的脂肪细胞的IR,其机制可能与H2S上调Glut4的表达有关。Objective To investigate the effect of extrogenous hydrogen sulfide （H2S） on the expression of glucose transporter 4 （Glut4） in 3T3-L1 adipocytes with insulin resistance and explore the mechanism. Methods 3T3-L1 adipocytes were incubated with high glucose and insulin to induce insulin resistance. The cells were treated with different concentrations of sodium bisulfide （NariS） （10-5, 10-4 and 10-3 mol/L） for 12, 24 and 48 h. MTT assay was used to detect the cell viability. The glucose consumption in the medium was measured by glucose oxidase method. The glucose uptake was tested by 2-deoxy-[ 3H ]-D-glucose method. The mRNA and protein expressions of Glut4 were measured by Real-time PCR and Western-Blot. Results Compared with control group, the glucose consumption and uptake and expressions of Glut4 were significantly decreased in the IR model group （ all P 〈 0. 05 ）. Compared with control group, NariS （ 10-5, 10-4 and 10-3 mol/L） did not affect the cell viability. Compared with model group, the glucose consumption and uptake and expressions of Glut4 were significantly increased by treatment with NariS （ 10 4 and 10-3 mol/L） for 24 and 48 h （all P 〈 0. 05）. Conclusions Extrogenous H2S improves IR induced by high glucose and insulin in 3T3-L1 adipocytes, and the mechanism may be related with up-regulation of Glut4 by H2S.

关 键 词：硫化氢 胰岛素抵抗 高糖 高胰岛素 葡萄糖转运体4型 

分 类 号：R96[医药卫生—药理学]