均匀设计法优化丹参中4种活性成分的超声提取工艺  

Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design

作　　者：郜舒蕊[1] 赵志刚[1] 侯俊玲[1] 王文全[1,2,3] 朱俊杰 王立会 韩亚男[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中药材规范化生产教育部工程研究中心,北京100102 [4]上海华宇药业有限公司,上海200002

出　　处：《中国实验方剂学杂志》2014年第10期8-12,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中药材规范化生产技术服务平台[工信部消费(2011)340];国家工业与信息部中药材生产扶持项目(丹参规范化;规模化和产业化生产基地建设)

摘　　要：目的：优选丹参中迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA共4种活性成分的超声提取工艺。方法：以迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA提取量为指标，采用均匀设计考察乙醇体积分数、液料比、提取时间对丹参提取效率的影响，通过逐步非线性回归优选提取工艺条件。利用HPLC测定指标成分含量，流动相甲醇（A）-0.01%磷酸水（B）梯度洗脱（0-3 min，30%A；3-5 min，30%-40%A；5-13 min，40%A；13-20 min，40%-58%A；20-22 min，58%-75%A；22-24 min，75%A；24-50 min，75%-85%A），检测波长286 nm（迷迭香酸和丹酚酸B）和268 nm（隐丹参酮和丹参酮ⅡA）。结果：最佳超声提取工艺条件为乙醇体积分数75%，液料比200 mLg-1，提取时间10 min；迷迭香酸、丹酚酸B、隐丹参酮及丹参酮ⅡA提取量分别为0.33，11.19，0.63，0.59 gg-1，与2010年版《中国药典》中加热回流法相比，活性成分提取量无显著差异。结论：该超声提取工艺具有提取时间短、低毒、无污染等优点。Objective： To optimize ultrasonic extraction technology of rosmarinic acid,salvianolic acid B,cryptotanshinone and tanshinone ⅡA from Salviae Miltiorrhizae Radix et Rhizoma. Method： With extraction amounts of four active components as indexes,uniform design was adopted to optimize extraction technology by taking ethanol concentration,solid-liquid ratio,extracting time as factors.HPLC was employed to determine contents of four active components with mobile phase of methanol-0.01% phosphoric acid solution（0-3 min,30%A;3-5 min,30%-40%A;5-13 min,40%A;13-20 min,40%-58%A;20-22 min,58%-75%A;22-24 min,75%A;24-50 min,75%-85%A） for gradient elution and detection wavelengths at 286 nm（rosmarinic acid and salvianolic acid B） and 268 nm（cryptotanshinone and tanshinone ⅡA）. Result： Optimum extraction conditions were as follows：ethanol concentration of 75%,liquid-solid ratio of 200 mLg-1,extraction time 10 min;Under these conditions,extraction amounts of rosmarinic acid,salvianolic acid B,cryptotanshinone and tanshinone ⅡA were 0.33,11.19,0.63 and 0.59 gg-1,respectively.Compared with heating reflux extraction in 2010 edition of ＂Chinese Pharmacopoeia＂,Contents of four bioactive components had no significant differences. Conclusion： This optimum extraction process had advantages of low mammalian toxicity,solvent saving,fast extracting speed and so on.

关 键 词：丹参 均匀设计 超声提取工艺 活性成分 迷迭香酸 丹酚酸B 隐丹参酮 丹参酮ⅡA 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]