正交试验优选江边一碗水的醋炙工艺  

Optimization of Processing Technology of Diphylleia sinensis Stir-baked with Vinegar by Orthogonal Test

作　　者：邓旭坤[1] 蒋捷[1] 林亲雄[1] 陈旅翼[1] 蔡爽[1] 吴非[1] 高康丽 张地[1] 张锦[1] 

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中国实验方剂学杂志》2014年第10期23-25,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十二五"科技支撑计划项目(2012BAI27B06);国家自然科学基金项目(81073147)

摘　　要：目的:优选江边一碗水的醋炙工艺。方法:以鬼臼毒素含量为指标,通过正交试验考察闷润时间、醋用量、炒制时间、炒制温度对醋炙工艺的影响。采用HPLC测定鬼臼毒素含量,流动相甲醇-0.1 moL·L-1磷酸二氢钾水溶液(45∶55),检测波长290 nm。结果:最佳炮制工艺为醋用量30%,闷润时间2 h,炒制温度150℃,炒制时间4 min;醋用量、炒制时间对醋炙工艺的影响具有显著性意义,鬼臼毒素质量分数达5.48%。结论:该炮制工艺合理可行,可为江边一碗水的民间安全用药提供参考。Objective： To optimize processing technology of Diphylleia sinensis stir-baked with vinegar. Method： Taking the content of podophyllotoxin as index,orthogonal test was adopted to optimize processing technology with four factors including moistening time,the amount of vinegar,frying time and temperature.With mobile phase of methanol-0.1 mol·L^-1 potassium dihydrogen phosphate solution（45∶55） and detection wavelength at 290 nm,the content of podophyllotoxin was determined by HPLC. Result： The best processing technology was as following：moistened 2 h with 30% vinegar,flew 4 min at 150 ℃.The amount of vinegar and frying time had significantly effects on processing technology,the mass fraction of podophyllotoxin was up to 5.48%. Conclusion： This optimized processing technology was reasonable and feasible.This result could provide a reference for medication safety of D. sinensis in folk.

关 键 词：醋炙法 江边一碗水 鬼臼毒素 正交试验 炮制工艺 

分 类 号：R283.3[医药卫生—中药学]