附子中生物碱含量与在胆巴液中浸泡时间变化规律的研究  

Study on Amount Variation of Alkaloids Components with Marinated Time in Aconiti Lateralis Radix Praeparata

作　　者：周林[1,2] 李飞[1] 任玉珍[2] 杜杰[2] 陈彦琳[2] 梁焕[2] 白宗利[2] 张晓莉 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国药材公司中药质量控制技术国家工程实验室,北京100195 [3]四川江油中坝附子科技发展有限公司,四川江油621700

出　　处：《中国实验方剂学杂志》2014年第10期44-47,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药行业科研专项(201107008)

摘　　要：目的:采用高效液相色谱法检测在胆巴溶液中浸泡不同时间的附子中6种生物碱的含量,分析附子中生物碱成分随浸泡时间的变化规律。方法:采用Waters sunfire C18色谱柱(4.6 mm×250 mm,5μm),流速0.8 mL·min-1,柱温30℃,检测波长235 nm,流动相乙腈-四氢呋喃(25∶15)和0.1 mol·L-1醋酸铵溶液(每1 000 mL加冰醋酸0.5 mL),梯度洗脱。结果:泥附子洗净后在胆巴溶液中浸泡,新乌头碱、次乌头碱、乌头碱和苯甲酰新乌头原碱的含量逐渐降低,浸泡20 d后,成分变化趋于平缓。浸泡20 d样品与生品比较,其降低率分别为60.39%,73.84%,72.57%和88.46%。结论:泥附子洗净后在胆巴溶液中浸泡,能达到防止腐烂的目的,浸泡20 d以后胆附子质量稳定,同时双酯型和单酯型生物碱总量均流失70%左右,为保证临床用药的安全和有效,有必要改进泥附子产地加工方法。Objective： To study the variations of different soaking marinated time on the alkaloids in aconite root, and the content of 6 alkaloids in different soaking marinated time samples of aconite root was analyzed by high performance liquid chromatography （HPLC）. Method： The content of alkaloids in aconite root was determined by HPLC with Waters Sunfire C_18 column （4.6 mm250 mm, 5 μm）. The mobile phase consisted of acetonitrile-tetrahydrofuran （25：15） and 0.1 mo·l^L-1 ammonium acetate solution （adding glacial acetic acid 0.5 mL into 1 000 mL solution） with the flow rate was 0.8 mL·min^-1. Detection wavelength was set at 235 nm. Column temperature was fixed at 30 ℃. Result： The content of mesaconitine, hypaconitine, aconitine and benzoylmesaconine in mud aconite decreased gradually when it was soaking in the Danba solution. After 20 days, compared with it before processing, the content of four compounds in processed aconite fell by 60.39%, 73.84%, 72.57% and 88.46% respectively. Conclusion： It can prevent mud aconite decaying when it was soaking in Danba solution. After 20 days, the quality of processed aconite was stable, and both of the content of diester-type alkaloids and monoester-type alkaloids fell by about 70%. In order to ensure the clinical medication safety and effectively, it is necessary to improve the processing method of mud aconite.

关 键 词：附子 胆巴溶液 新乌头碱 次乌头碱 乌头碱 苯甲酰新乌头原碱 

分 类 号：R284.1[医药卫生—中药学]