UPLC测定香鳞毛蕨中绵马素BB的含量  

Determination of Aspidin BB in Dryopteris fragrans by UPLC

作　　者：汤文伟 沈志滨[1] 尹永芹[1] 吴洁莹[1] 张慧[1] 黄永昌[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《中国实验方剂学杂志》2014年第10期51-53,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"重大新药创制专项(2011zx09102-007-03);国家自然科学基金项目(81202885);广东省科技计划项目(2011A030100013);广州市科技计划项目(12A062131631);广东省教育厅高层次人才项目(2010)

摘　　要：目的：建立超高效液相色谱法测定香鳞毛蕨药材中绵马素 BB的含量。方法：采用XBridge^TM BEH C_18（4.6 mm100 mm，2.5 μm）色谱柱，以乙腈-0.1%三氟乙酸水溶液（90∶10）为流动相，流速0.80 mL·min^-1，检测波长290 nm，柱温40 ℃，进行测定。结果：绵马素BB在0.050 28-0.301 68 μg线性关系良好（r=0.999 8），平均回收率为98.87%（RSD 0.44%）。结论：该方法快速、准确、重复性好，可用于香鳞毛蕨的含量测定和质量控制。Objective： To establish an UPLC method for the content determination of aspidin BB in Dryopteris fragrans. Method： Determination was performed on XBridge^TM BEH C_18 column （4.6 mm100 mm,2.5 μm） with mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid with detection wavelength at 290 nm. The flow rate was set to 0.8 mL·min^-1,while the column temperature was set at 40 ℃. Result： The linear range of aspidin BB was 0.050 28-0.301 68 μg（r=0.999 8）with an average recovery of 98.87%（RSD 0.44%,n=6）. Conclusion： The method is fast,accurate and reproducible, which can be used to control the quality of D. fragrans.

关 键 词：超高液相色谱 香鳞毛蕨 绵马素 BB 含量测定 

分 类 号：R284.1[医药卫生—中药学]